MicroRNA-146a regulates cisplatin-resistance of non-small cell lung cancer cells by targeting NF-κB pathway.

Autor:	Jiang P; Department of Thoracic Surgery, Second Hospital of Lanzhou University Lanzhou 730030, Gansu Province, China., Jia W; Department of Pathology, Shenzhen Hospital of Southern Medical University Shenzhen, China., Wei X; Department of Thoracic Surgery, Second Hospital of Lanzhou University Lanzhou 730030, Gansu Province, China., Zhang X; Department of Outpatient, Second Hospital of Lanzhou University Lanzhou 730030, Gansu Province, China., Wang C; Department of Thoracic Surgery, Second Hospital of Lanzhou University Lanzhou 730030, Gansu Province, China., Li B; Department of Thoracic Surgery, Second Hospital of Lanzhou University Lanzhou 730030, Gansu Province, China., Song T; Department of Thoracic Surgery, Second Hospital of Lanzhou University Lanzhou 730030, Gansu Province, China., Yang J; Department of Thoracic Surgery, Second Hospital of Lanzhou University Lanzhou 730030, Gansu Province, China., Zhu D; Department of Thoracic Surgery, Second Hospital of Lanzhou University Lanzhou 730030, Gansu Province, China., Meng Y; Department of Thoracic Surgery, Second Hospital of Lanzhou University Lanzhou 730030, Gansu Province, China.
Jazyk:	angličtina
Zdroj:	International journal of clinical and experimental pathology [Int J Clin Exp Pathol] 2017 Dec 01; Vol. 10 (12), pp. 11545-11553. Date of Electronic Publication: 2017 Dec 01 (Print Publication: 2017).
Abstrakt:	The aim of this study is to explore the influence of miR-146a on cisplatin resistance in non-small cell lung cancer (NSCLC) cells and the related molecular mechanism. The expression of miR-146a in NSCLC tumor samples and cell lines was measured by qRT-PCR. The DDP (cisplatin) cytotoxicity was detected by CCK-8 assay. The protein expressions of TRAF6, IRAK1, p50, p-p65, p65 in normal DDP-resistant cells were determined by western blot analysis. Luciferase reporter assay was used to investigate the relationship between miR-146a and NF-κB pathway activity. The expression of miR-146a in DDP-resistant NSCLC tumor samples was significantly lower than that in DDP-sensitive ones. Its expression in DDP-resistant cell lines was much lower as well. The protein levels of TRAF6, IRAK1 and p50 were up-regulated in A549/DDP and Calu-1/DDP cells compared to parental cells, and phosphorylation of p65 was also increased, indicating the activation of NF-κB signaling pathway. Furthermore, NF-κB activity was conversely related with miR-146a level in NSCLC. It was revealed that miR-146a expression in NSCLC was negatively correlated with activation of of NF-κB pathway. In conclusion, the study indicates that miR-146a regulates the DDP sensitivity by inhibiting NF-κB signaling pathway in NSCLC cells. Competing Interests: None. (IJCEP Copyright © 2017.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu