Residential exposure to radon and levels of histone γH2AX and DNA damage in peripheral blood lymphocytes of residents of Kowary city regions (Poland).

Autor: K W; Radiological Protection Dept, Nofer Institute of Occupational Medicine, ul. Św. Teresy od Dzieciątka Jezus 8, 91-348, Łódź, Poland. Electronic address: Katarzyna.Walczak@imp.lodz.pl., J O; Radiological Protection Dept, Nofer Institute of Occupational Medicine, ul. Św. Teresy od Dzieciątka Jezus 8, 91-348, Łódź, Poland., P P; Radiological Protection Dept, Nofer Institute of Occupational Medicine, ul. Św. Teresy od Dzieciątka Jezus 8, 91-348, Łódź, Poland., K DG; Toxicology and Carcinogenesis Dept, Nofer Institute of Occupational Medicine, ul. Św. Teresy od Dzieciątka Jezus 8, 91-348, Łódź, Poland., K K; Toxicology and Carcinogenesis Dept, Nofer Institute of Occupational Medicine, ul. Św. Teresy od Dzieciątka Jezus 8, 91-348, Łódź, Poland., M Z; Radiological Protection Dept, Nofer Institute of Occupational Medicine, ul. Św. Teresy od Dzieciątka Jezus 8, 91-348, Łódź, Poland., M B; Radiological Protection Dept, Nofer Institute of Occupational Medicine, ul. Św. Teresy od Dzieciątka Jezus 8, 91-348, Łódź, Poland., M S; Toxicology and Carcinogenesis Dept, Nofer Institute of Occupational Medicine, ul. Św. Teresy od Dzieciątka Jezus 8, 91-348, Łódź, Poland.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2020 May; Vol. 247, pp. 125748. Date of Electronic Publication: 2019 Dec 24.
DOI: 10.1016/j.chemosphere.2019.125748
Abstrakt: Introduction: Radon-induced biological effects have been studied mainly through epidemiological investigations, and well-controlled in vitro and in vivo experiments. To provide data explaining radon exposure-induced harmful effects in natural environment, exposure assessment under these conditions is needed. The objective of the study was to examine the level of genetic damage assessed with biomarkers of DNA single- and double-strand breaks (SSBs and DSBs) in peripheral blood mononuclear cells obtained from individuals continuously exposed to Rn in homes. Naturally elevated Rn concentrations in homes can be found in the South of Poland, in Kowary city.
Methods: Measurements of expression of phosphorylated histone γH2AX was used as a marker of DNA double strand breaks. To detect DNA single and double-strand breaks and alkali labile sites, the alkaline comet assay was used. Oxidative damage of DNA was evaluated by formamidopyrimidyne (FPG)-modified comet assay. The blood was collected from 94 volunteers living in Kowary. Subjects were grouped according to their status of living in radon concentration ≥100 Bq/m 3 (n = 67), and <100 Bq/m 3 (n = 27).
Results: The statistically significant differences in levels of DNA damage in peripheral lymphocytes assessed with comet assay were found to be associated with levels of radon exposure in indoor air (p = 0.034). DNA damage in the comet assay was significantly correlated with DNA damage assessed with γH2AX staining.
Conclusions: Results of the present study indicate the suitability of alkaline comet assay for the detection of DNA damage in peripheral blood lymphocytes of people environmentally exposed to radon.
Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest.
(Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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