Investigating the Potential and Pitfalls of EV-Encapsulated MicroRNAs as Circulating Biomarkers of Breast Cancer.

Autor:	Moloney BM; Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, National University of Ireland Galway, Galway, H91 YR71, Ireland., Gilligan KE; Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, National University of Ireland Galway, Galway, H91 YR71, Ireland., Joyce DP; Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, National University of Ireland Galway, Galway, H91 YR71, Ireland., O'Neill CP; Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, National University of Ireland Galway, Galway, H91 YR71, Ireland., O'Brien KP; Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, National University of Ireland Galway, Galway, H91 YR71, Ireland., Khan S; Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, National University of Ireland Galway, Galway, H91 YR71, Ireland., Glynn CL; Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, National University of Ireland Galway, Galway, H91 YR71, Ireland., Waldron RM; Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, National University of Ireland Galway, Galway, H91 YR71, Ireland., Maguire CM; Laboratory for Biological Characterisation of Advanced Materials (LBCAM) and Advanced Materials and Bioengineering Research (AMBER) Centre, Trinity Translational Medicine Institute, Trinity College Dublin, James Street, Dublin, D08 W9RT, Ireland., Holian E; School of Mathematics, Statistics and Applied Mathematics, National University of Ireland Galway, Galway, H91 YR71, Ireland., Naughton E; Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, National University of Ireland Galway, Galway, H91 YR71, Ireland., Elhadi M; Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, National University of Ireland Galway, Galway, H91 YR71, Ireland., Grealish AB; Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, National University of Ireland Galway, Galway, H91 YR71, Ireland., Malone C; Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, National University of Ireland Galway, Galway, H91 YR71, Ireland., McDermott E; Centre for Microscopy and Imaging, Discipline of Anatomy, School of Medicine, National University of Ireland Galway, Galway, H91 YR71, Ireland., Dockery P; Centre for Microscopy and Imaging, Discipline of Anatomy, School of Medicine, National University of Ireland Galway, Galway, H91 YR71, Ireland., Ritter T; Regenerative Medicine Institute, School of Medicine, College of Medicine, Nursing and Health Sciences, National University of Ireland Galway, Galway, H91 YR71, Ireland., Prina-Mello A; Laboratory for Biological Characterisation of Advanced Materials (LBCAM) and Advanced Materials and Bioengineering Research (AMBER) Centre, Trinity Translational Medicine Institute, Trinity College Dublin, James Street, Dublin, D08 W9RT, Ireland., Kerin MJ; Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, National University of Ireland Galway, Galway, H91 YR71, Ireland., Dwyer RM; Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, National University of Ireland Galway, Galway, H91 YR71, Ireland.
Jazyk:	angličtina
Zdroj:	Cells [Cells] 2020 Jan 07; Vol. 9 (1). Date of Electronic Publication: 2020 Jan 07.
DOI:	10.3390/cells9010141
Abstrakt:	Extracellular vesicles (EVs) shuttle microRNA (miRNA) throughout the circulation and are believed to represent a fingerprint of the releasing cell. We isolated and characterized serum EVs of breast tumour-bearing animals, breast cancer (BC) patients, and healthy controls. EVs were characterized using transmission electron microscopy (TEM), protein quantification, western blotting, and nanoparticle tracking analysis (NTA). Absolute quantitative (AQ)-PCR was employed to analyse EV-miR-451a expression. Isolated EVs had the appropriate morphology and size. Patient sera contained significantly more EVs than did healthy controls. In tumour-bearing animals, a correlation between serum EV number and tumour burden was observed. There was no significant relationship between EV protein yield and EV quantity determined by NTA, highlighting the requirement for direct quantification. Using AQ-PCR to relate miRNA copy number to EV yield, a significant increase in miRNA-451a copies/EV was detected in BC patient sera, suggesting potential as a novel biomarker of breast cancer. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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