Mega-plasmid found worldwide confers multiple antimicrobial resistance in Salmonella Infantis of broiler origin in Russia.

Autor: Bogomazova AN; The Russian State Center for Animal Feed and Drug Standardization and Quality (FGBU 'VGNKI'), Zvenigorodskoe shosse 5, Moscow 132022, Russia. Electronic address: sequence@vgnki.ru., Gordeeva VD; The Russian State Center for Animal Feed and Drug Standardization and Quality (FGBU 'VGNKI'), Zvenigorodskoe shosse 5, Moscow 132022, Russia., Krylova EV; The Russian State Center for Animal Feed and Drug Standardization and Quality (FGBU 'VGNKI'), Zvenigorodskoe shosse 5, Moscow 132022, Russia., Soltynskaya IV; The Russian State Center for Animal Feed and Drug Standardization and Quality (FGBU 'VGNKI'), Zvenigorodskoe shosse 5, Moscow 132022, Russia., Davydova EE; The Russian State Center for Animal Feed and Drug Standardization and Quality (FGBU 'VGNKI'), Zvenigorodskoe shosse 5, Moscow 132022, Russia., Ivanova OE; The Russian State Center for Animal Feed and Drug Standardization and Quality (FGBU 'VGNKI'), Zvenigorodskoe shosse 5, Moscow 132022, Russia., Komarov AA; The Russian State Center for Animal Feed and Drug Standardization and Quality (FGBU 'VGNKI'), Zvenigorodskoe shosse 5, Moscow 132022, Russia.
Jazyk: angličtina
Zdroj: International journal of food microbiology [Int J Food Microbiol] 2020 Apr 16; Vol. 319, pp. 108497. Date of Electronic Publication: 2019 Dec 26.
DOI: 10.1016/j.ijfoodmicro.2019.108497
Abstrakt: Plasmids which are the mobile part of the bacterial genome can acquire and carry over genes conferring antimicrobial resistance, thus contributing to rapid adaptation of bacterial community to human-defined environment. In 2014, Israeli scientists have reported a large conjugative mega-plasmid pESI (plasmid for emerging S. Infantis) that provides multiple drug resistance (MDR) of Salmonella Infantis isolated from broilers. Later, very similar pESI-like plasmids have been found in Salmonella isolated from poultry in the United States, Italy, Switzerland, Hungary, and Japan. Here we report detection of pESI-like plasmids in Salmonella Infantis isolated from chicken food products in Russia. Whole genome sequencing of three MDR isolates revealed pESI-like plasmids in all three cases. These plasmids have such typical pESI features as a locus for siderophore yersiniabactin, a cluster of IncI1 conjugative genes, a cluster of type IV pilus genes, and three toxin-antitoxin modules. The pESI-like plasmids carry from two to five resistance genes in each isolate. In total, we observed six antimicrobial resistance genes associated with pESI-like plasmids (aadA1, bla CTX-M-14 , dfrA14, sul1, tetA/tetR, tetM). Besides plasmid genes of antimicrobial resistance, all three MDR isolates of S. Infantis harbor a mutation in chromosomal gene gyrA (p.S83Y or p.D87Y) that is associated with resistance to fluoroquinolones. In addition, we performed a comparative bioinformatics meta-analysis of 25 pESI-like plasmids hosted by S. Infantis from the USA, Europe, Latin America, Israel, and Japan. This analysis identified a 173 kB sequence that is common for all pESI-like plasmids and carries virulence operons and toxin-antitoxin modules.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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