Polymorphic Protective Dps-DNA Co-Crystals by Cryo Electron Tomography and Small Angle X-Ray Scattering.

Autor: Kamyshinsky R; National Research Center 'Kurchatov Institute', Akademika Kurchatova pl., 1, 123182 Moscow, Russia.; Shubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia.; Moscow Institute of Physics and Technology, Institutsky lane 9, 141700 Dolgoprudny, Moscow Region, Russia., Chesnokov Y; National Research Center 'Kurchatov Institute', Akademika Kurchatova pl., 1, 123182 Moscow, Russia.; Shubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia., Dadinova L; Shubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia., Mozhaev A; Shubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia.; Shemyakin-Ovchinnikov Institute of bioorganic chemistry of Russian Academy of Sciences, Miklukho-Maklaya, 16/10, 117997 Moscow, Russia., Orlov I; Shubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia., Petoukhov M; Shubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia.; Frumkin Institute of Physical Chemistry and Electrochemistry of Russian Academy of Sciences, Leninsky prospect, 31, 119071 Moscow, Russia., Orekhov A; National Research Center 'Kurchatov Institute', Akademika Kurchatova pl., 1, 123182 Moscow, Russia.; Shubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia.; Moscow Institute of Physics and Technology, Institutsky lane 9, 141700 Dolgoprudny, Moscow Region, Russia., Shtykova E; Shubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia., Vasiliev A; National Research Center 'Kurchatov Institute', Akademika Kurchatova pl., 1, 123182 Moscow, Russia.; Shubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences, Leninskiy prospect, 59, 119333 Moscow, Russia.; Moscow Institute of Physics and Technology, Institutsky lane 9, 141700 Dolgoprudny, Moscow Region, Russia.
Jazyk: angličtina
Zdroj: Biomolecules [Biomolecules] 2019 Dec 26; Vol. 10 (1). Date of Electronic Publication: 2019 Dec 26.
DOI: 10.3390/biom10010039
Abstrakt: Rapid increase of intracellular synthesis of specific histone-like Dps protein that binds DNA to protect the genome against deleterious factors leads to in cellulo crystallization-one of the most curious processes in the area of life science at the moment. However, the actual structure of the Dps-DNA co-crystals remained uncertain in the details for more than two decades. Cryo-electron tomography and small-angle X-ray scattering revealed polymorphous modifications of the co-crystals depending on the buffer parameters. Two different types of the Dps-DNA co-crystals are formed in vitro: triclinic and cubic. Three-dimensional reconstruction revealed DNA and Dps molecules in cubic co-crystals, and the unit cell parameters of cubic lattice were determined consistently by both methods.
Databáze: MEDLINE