Islet Harvest in Carbon Monoxide-Saturated Medium for Chronic Pancreatitis Patients Undergoing Islet Autotransplantation.

Autor:	Wang H; Department of Surgery, Medical University of South Carolina, Charleston, SC, USA., Gou W; Department of Surgery, Medical University of South Carolina, Charleston, SC, USA., Strange C; Department of Medicine, Medical University of South Carolina, Charleston, SC, USA., Wang J; Department of Surgery, Medical University of South Carolina, Charleston, SC, USA., Nietert PJ; Department of Public Health Sciences, Medical University of South Carolina, Charleston, SC, USA., Cloud C; Department of Surgery, Medical University of South Carolina, Charleston, SC, USA., Owzarski S; Department of Surgery, Medical University of South Carolina, Charleston, SC, USA., Shuford B; Department of Surgery, Medical University of South Carolina, Charleston, SC, USA., Duke T; Department of Surgery, Medical University of South Carolina, Charleston, SC, USA., Luttrell L; Department of Medicine, Medical University of South Carolina, Charleston, SC, USA., Lesher A; Department of Surgery, Medical University of South Carolina, Charleston, SC, USA., Papas KK; Department of Surgery, University of Arizona, Tucson, AZ, USA., Herold KC; Department of Immunology, Yale University, New Haven, CT, USA., Clark P; Department of Immunology, Yale University, New Haven, CT, USA., Usmani-Brown S; Department of Immunology, Yale University, New Haven, CT, USA., Kitzmann J; Department of Surgery, University of Arizona, Tucson, AZ, USA., Crosson C; Department of Medicine, Medical University of South Carolina, Charleston, SC, USA., Adams DB; Department of Surgery, Medical University of South Carolina, Charleston, SC, USA., Morgan KA; Department of Surgery, Medical University of South Carolina, Charleston, SC, USA.
Jazyk:	angličtina
Zdroj:	Cell transplantation [Cell Transplant] 2019 Dec; Vol. 28 (1_suppl), pp. 25S-36S. Date of Electronic Publication: 2019 Dec 30.
DOI:	10.1177/0963689719890596
Abstrakt:	Stresses encountered during human islet isolation lead to unavoidable β-cell death after transplantation. This reduces the chance of insulin independence in chronic pancreatitis patients undergoing total pancreatectomy and islet autotransplantation. We tested whether harvesting islets in carbon monoxide-saturated solutions is safe and can enhance islet survival and insulin independence after total pancreatectomy and islet autotransplantation. Chronic pancreatitis patients who consented to the study were randomized into carbon monoxide (islets harvested in a carbon monoxide-saturated medium) or control (islets harvested in a normal medium) groups. Islet yield, viability, oxygen consumption rate, β-cell death (measured by unmethylated insulin DNA), and serum cytokine levels were measured during the peri-transplantation period. Adverse events, metabolic phenotypes, and islet function were measured prior and at 6 months post-transplantation. No adverse events directly related to the infusion of carbon monoxide islets were observed. Carbon monoxide islets showed significantly higher viability before transplantation. Subjects receiving carbon monoxide islets had less β-cell death, decreased CCL23, and increased CXCL12 levels at 1 or 3 days post transplantation compared with controls. Three in 10 (30%) of the carbon monoxide subjects and none of the control subjects were insulin independent. This pilot trial showed for the first time that harvesting human islets in carbon monoxide-saturated solutions is safe for total pancreatectomy and islet autotransplantation patients.
Databáze:	MEDLINE
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