| Autor: |
Stanton RH; Department of Zoology, University of Georgia, Athens 30602., Plapp FW Jr, White RA, Agosin M |
| Jazyk: |
angličtina |
| Zdroj: |
Comparative biochemistry and physiology. B, Comparative biochemistry [Comp Biochem Physiol B] 1978; Vol. 61 (2), pp. 297-305. |
| DOI: |
10.1016/0305-0491(78)90179-7 |
| Abstrakt: |
1. Microsomal fractions isolated from various housefly strains have been characterized with respect to multiple forms of cytochrome P-450 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 2. Susceptible NAIDM houseflies were pretreated with known inducers of cytochrome P-450, and their microsomal electrophoretic profiles were compared to control NAIDM microsomes, using as standards partially purified cytochrome P-450s from noninduced NAIDM houseflies. 3. Tentatively, at least five different species of cytochrome P-450 may exist in the NAIDM housefly strain. 4. A comparison of the microsomal electrophoretic profile of different housefly strains also indicates the presence of at least two additional cytochrome P-450 species. 5. Induction with alpha-pinene and phenobarbital was expressed by a shift of the maximum absorbance at 452 nm in the CO-difference spectrum to lower wavelengths in the NAIDM strain; whereas, beta-naphthoflavone, although increasing the amount of cytochrome P-450, did not change the wavelength of maximum absorbance. 6. Cytochromes of the P-452 type appear to predominate in the susceptible NAIDM strain, while cytochromes of the P-450 and P-448 types predominate in resistant strains. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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