Response of preantral follicles exposed to quinoxaline: A new compound with anticancer potential.

Autor: Guerreiro DD; Laboratory of Manipulation of Oocyte and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary (FAVET), State University of Ceará, Fortaleza, Brazil., de Lima LF; Laboratory of Manipulation of Oocyte and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary (FAVET), State University of Ceará, Fortaleza, Brazil., de Sá NAR; Laboratory of Manipulation of Oocyte and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary (FAVET), State University of Ceará, Fortaleza, Brazil., Mbemya GT; Laboratory of Manipulation of Oocyte and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary (FAVET), State University of Ceará, Fortaleza, Brazil., Ferreira ACA; Laboratory of Manipulation of Oocyte and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary (FAVET), State University of Ceará, Fortaleza, Brazil., Alves BG; Laboratory of Biology of Reproduction, Federal University of Uberlândia, Minas Gerais, Brazil., Sant'anna Maranhão S; Department Pharmacology, Federal University of Ceará, Fortaleza, CE 60430-270, Brazil., do Ó Pessoa C; Department Pharmacology, Federal University of Ceará, Fortaleza, CE 60430-270, Brazil., Pinheiro AC; Instituto de Tecnologia em Fármacos-Far Manguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil., Nogueira TCM; Instituto de Tecnologia em Fármacos-Far Manguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil., de Souza MVN; Instituto de Tecnologia em Fármacos-Far Manguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil., de Figueiredo JR; Laboratory of Manipulation of Oocyte and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary (FAVET), State University of Ceará, Fortaleza, Brazil., Rodrigues APR; Laboratory of Manipulation of Oocyte and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary (FAVET), State University of Ceará, Fortaleza, Brazil. Electronic address: denisedguerreiro4@gmai.com.
Jazyk: angličtina
Zdroj: Research in veterinary science [Res Vet Sci] 2020 Feb; Vol. 128, pp. 261-268. Date of Electronic Publication: 2019 Dec 05.
DOI: 10.1016/j.rvsc.2019.12.010
Abstrakt: The culture of preantral follicles as an in vitro model to evaluate the toxicity of new anticancer drug has being established. Therefore, the aim of this study was to evaluate the effect of quinoxaline derivative the 2 2- (XYZC 6H 3 -CH=N-NH)-quinoxaline, 1 (QX) on caprine preantral follicles. We evaluate the follicular morphology and activation, proliferation and apoptosis of granulosa cells and finally the protein (ABCB1) and genes expression (cyclin/Cdks), respectively involved in multidrug resistance and cell cycle progression. Ovarian fragments containing primordial and developing follicles were exposed (in vitro culture) to different concentrations of QX (QX1.5, QX3.0 or QX6.0 μM/mL) during 6 days. To evaluate the effect of QX, the ovarian tissue was exposed to Paclitaxel 0.1 μg/mL (PTX - negative control) or in culture media without QX (MEM). At the end of exposure time, we realized that the QX (all concentrations) increased (P < .05) the normal morphology of preantral follicles compared to control (not treated ovarian tissue) or MEM. However, QX6.0 showed a enhanced (P < .05) on follicular activation (burnout) and apoptosis than QX1.5 and QX3.0. Expression of ABCB1 was similar between QX1.5 and QX6.0 and both were lower than control, MEM and PTX. Interestingly, the apoptosis rate in QX3.0 was similar to control and MEM and lower then QX1.5; QX6.0 and PTX. We conclude that quinoxaline may be a promising chemotherapeutic agent, however, other concentrations within a defined range (2-5.5 μM) could be widely investigated.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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