The Human Cytomegalovirus Nonstructural Glycoprotein UL148 Reorganizes the Endoplasmic Reticulum.

Autor:	Zhang H; Department of Microbiology and Immunology, LSU Health Sciences Center, Shreveport, Louisiana, USA., Read C; Institute of Virology, Ulm University Medical Center, Ulm, Germany.; Central Facility for Electron Microscopy, Ulm University, Ulm, Germany., Nguyen CC; Department of Microbiology and Immunology, LSU Health Sciences Center, Shreveport, Louisiana, USA., Siddiquey MNA; Department of Microbiology and Immunology, LSU Health Sciences Center, Shreveport, Louisiana, USA., Shang C; Research Core Facility, LSU Health Sciences Center, Shreveport, Louisiana, USA., Hall CM; Department of Microbiology and Immunology, LSU Health Sciences Center, Shreveport, Louisiana, USA., von Einem J; Institute of Virology, Ulm University Medical Center, Ulm, Germany., Kamil JP; Department of Microbiology and Immunology, LSU Health Sciences Center, Shreveport, Louisiana, USA jkamil@lsuhsc.edu.; Center for Molecular and Tumor Virology, LSU Health Sciences Center, Shreveport, Louisiana, USA.
Jazyk:	angličtina
Zdroj:	MBio [mBio] 2019 Dec 10; Vol. 10 (6). Date of Electronic Publication: 2019 Dec 10.
DOI:	10.1128/mBio.02110-19
Abstrakt:	Human cytomegalovirus (HCMV) encodes an endoplasmic reticulum (ER)-resident glycoprotein, UL148, which activates the unfolded protein response (UPR) but is fully dispensable for viral replication in cultured cells. Hence, its previously ascribed roles in immune evasion and modulation of viral cell tropism are hypothesized to cause ER stress. Here, we show that UL148 is necessary and sufficient to drive the formation of prominent ER-derived structures that on average occupy 5% of the infected cell cytoplasm. The structures are sites where UL148 coalesces with cellular proteins involved in ER quality control, such as HRD1 and EDEM1. Electron microscopy revealed that cells infected with wild-type but not UL148 -null HCMV show prominent accumulations of densely packed ruffled ER membranes which connect to distended cisternae of smooth and partially rough ER. During ectopic expression of UL148-green fluorescent protein (GFP) fusion protein, punctate signals traffic to accumulate at conspicuous structures. The structures exhibit poor recovery of fluorescence after photobleaching, which suggests that their contents are poorly mobile and do not efficiently exchange with the rest of the ER. Small-molecule blockade of the integrated stress response (ISR) prevents the formation of puncta, leading to a uniform reticular fluorescent signal. Accordingly, ISR inhibition during HCMV infection abolishes the coalescence of UL148 and HRD1 into discrete structures, which argues that UL148 requires the ISR to cause ER reorganization. Given that UL148 stabilizes immature forms of a receptor binding subunit for a viral envelope glycoprotein complex important for HCMV infectivity, our results imply that stress-dependent ER remodeling contributes to viral cell tropism. IMPORTANCE Perturbations to endoplasmic reticulum (ER) morphology occur during infection with various intracellular pathogens and in certain genetic disorders. We identify that a human cytomegalovirus (HCMV) gene product, UL148, profoundly reorganizes the ER during infection and is sufficient to do so when expressed on its own. Our results reveal that UL148-dependent reorganization of the ER is a prominent feature of HCMV-infected cells. Moreover, we find that this example of virally induced organelle remodeling requires the integrated stress response (ISR), a stress adaptation pathway that contributes to a number of disease states. Since ER reorganization accompanies roles of UL148 in modulation of HCMV cell tropism and in evasion of antiviral immune responses, our results may have implications for understanding the mechanisms involved. Furthermore, our findings provide a basis to utilize UL148 as a tool to investigate organelle responses to stress and to identify novel drugs targeting the ISR. (Copyright © 2019 Zhang et al.)
Databáze:	MEDLINE
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