Effects of exercise training and stem cell therapy on the left ventricle of infarcted rats.

Autor:	de Freitas JS; General Biology Department, Universidade Federal de Viçosa (UFV), Viçosa, MG, Brazil; Physical Education Department, Universidade Federal de Viçosa (UFV), Viçosa, MG, Brazil., Neves CA; General Biology Department, Universidade Federal de Viçosa (UFV), Viçosa, MG, Brazil., Del Carlo RJ; Veterinary Medicine Department, Universidade Federal de Viçosa (UFV), Viçosa, MG, Brazil., Belfort FG; Physical Education Department, Universidade Federal de Viçosa (UFV), Viçosa, MG, Brazil., Lavorato VN; Physical Education Department, Universidade Federal de Viçosa (UFV), Viçosa, MG, Brazil., Silame-Gomes LHL; Physical Education Department, Universidade Federal de Viçosa (UFV), Viçosa, MG, Brazil., Ramos RMS; Physical Education Department, Universidade Federal de Viçosa (UFV), Viçosa, MG, Brazil., Cunha DQND; Physical Education Department, Universidade Federal de Viçosa (UFV), Viçosa, MG, Brazil., Okano BS; Veterinary Medicine Department, Universidade Federal de Viçosa (UFV), Viçosa, MG, Brazil., Pereira VG; Veterinary Medicine Department, Universidade Federal de Viçosa (UFV), Viçosa, MG, Brazil., de Oliveira EM; School of Physical Education and Sport, Universidade de São Paulo (USP), São Paulo, SP, Brazil., Carneiro-Júnior MA; Physical Education Department, Universidade Federal de Viçosa (UFV), Viçosa, MG, Brazil., Natali AJ; Physical Education Department, Universidade Federal de Viçosa (UFV), Viçosa, MG, Brazil. Electronic address: anatali@ufv.br.
Jazyk:	English; Portuguese
Zdroj:	Revista portuguesa de cardiologia [Rev Port Cardiol (Engl Ed)] 2019 Sep; Vol. 38 (9), pp. 649-656. Date of Electronic Publication: 2019 Dec 05.
DOI:	10.1016/j.repc.2019.02.013
Abstrakt:	Introduction and Objectives: Stem cell therapy and aerobic exercise are non-pharmacological therapies following myocardial infarction. The aim of this study was to test whether aerobic exercise training enhances the benefits of mesenchymal stem cell (MSC) therapy on remodeling of the extracellular matrix and fetal gene expression in the left ventricle of infarcted rats. Methods: Myocardial infarction was surgically induced in six-week old male Wistar rats. Animals were divided into four groups: sedentary control (SC) and sedentary and stem cell treated (SCMSC); exercised (EX) and exercised and stem cell treated (EXMSC). Bone marrow-derived MSCs were immediately transplanted via the tail vein (concentration: 1×10 6 cells). Exercise training (five days/week, 60 min/day; 60% of maximal running speed) started 24 hours after myocardial infarction and lasted for 12 weeks. Results: Exercise capacity was higher in exercised than in sedentary groups. Animals in the SCMSC, EX and EXMSC groups exhibited better cardiac function than those in SC. Collagen content was lower in the SCMSC, EX and EXMSC groups than in SC and skeletal α-actin expression was lower in EX and EXMSC than in SC. The α/β-MHC ratio was higher in EX and EXMSC than in SC. The combination of therapies further reduced collagen content in the remote region of the infarct (∼24%) and skeletal α-actin expression (∼30%). Conclusion: Aerobic exercise training appears to enhance the beneficial effects of stem cell therapy on remodeling of the extracellular matrix and fetal gene expression in the left ventricle of rats with moderate infarction. (Copyright © 2019 Sociedade Portuguesa de Cardiologia. Publicado por Elsevier España, S.L.U. All rights reserved.)
Databáze:	MEDLINE
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