QUERCETIN SUPPLEMENTATION PREVENTS CHANGES IN THE SEROTONIN AND CASPASE-3 IMMUNOREACTIVE CELLS OF THE JEJUNUM OF DIABETIC RATS.

Autor:	Martins-Perles JVC; Universidade Estadual de Maringá, Departamento de Ciências Morfológicas - Laboratório de Plasticidade Neural Entérica, Maringá, PR, Brasil., Zignani I; Universidade Estadual de Maringá, Departamento de Ciências Morfológicas - Laboratório de Plasticidade Neural Entérica, Maringá, PR, Brasil., Souza SRG; Universidade Estadual de Maringá, Departamento de Ciências Morfológicas - Laboratório de Plasticidade Neural Entérica, Maringá, PR, Brasil., Frez FCV; Universidade Estadual de Maringá, Departamento de Ciências Morfológicas - Laboratório de Plasticidade Neural Entérica, Maringá, PR, Brasil., Bossolani GDP; Universidade Estadual de Maringá, Departamento de Ciências Morfológicas - Laboratório de Plasticidade Neural Entérica, Maringá, PR, Brasil., Zanoni JN; Universidade Estadual de Maringá, Departamento de Ciências Morfológicas - Laboratório de Plasticidade Neural Entérica, Maringá, PR, Brasil.
Jazyk:	angličtina
Zdroj:	Arquivos de gastroenterologia [Arq Gastroenterol] 2019 Oct-Dec; Vol. 56 (4), pp. 405-411.
DOI:	10.1590/S0004-2803.201900000-81
Abstrakt:	Background: Serotonin (5-HT) is present in the epithelial enterochromaffin cells (EC), mast cells of the lamina propria and enteric neurons. The 5-HT is involved in regulating motility, secretion, gut sensation, immune system and inflammation. Objective: Evaluate the effects of diabetes and quercetin supplementation on serotoninergic cells and its cell loss by apoptosis in jejunal mucosa of streptozotocin-induced diabetic rats (STZ-rats). Methods: Twenty-four male Wistar rats were divided into four groups: normoglycemic (C), normoglycemic supplemented with 40 mg/day quercetin (Q), diabetic (D) and diabetic supplemented with 40 mg/day quercetin (DQ). After 120 days, the jejunum was collected and fixated in Zamboni's solution for 18 h. After obtaining cryosections, immunohistochemistry was performed to label 5-HT and caspase-3. Quantification of 5-HT and caspase-3 immunoreactive (IR) cells in the lamina propria, villi and crypts were performed. Results: The diabetic condition displayed an increase of the number of 5-HT-IR cells in villi and crypts, while decreased number of these cells was observed in lamina propria in the jejunum of STZ-rats. In the diabetic animals, an increased density of apoptotic cells in epithelial villi and crypts of the jejunum was observed, whereas a decreased number of caspase-3-IR cells was observed in lamina propria. Possibly, quercetin supplementation slightly suppressed the apoptosis phenomena in the epithelial villi and crypts of the STZ-rats, however the opposite effect was observed on the 5-HT-IR cells of the lamina propria. Quercetin supplementation on healthy animals promoted few changes of serotoninergic function and apoptotic stimuli. Conclusion: These results suggest that quercetin supplementation mostly improved the serotonergic function affected by diabetes maybe due to antioxidant and anti-inflammatory properties of quercetin.
Databáze:	MEDLINE
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