Schinus terebinthifolia leaf lectin (SteLL) has anti-infective action and modulates the response of Staphylococcus aureus-infected macrophages.

Autor: de Souza Feitosa Lima IM; Programas de Pós-Graduação, Universidade Ceuma, São Luís, Maranhão, Brazil., Zagmignan A; Programas de Pós-Graduação, Universidade Ceuma, São Luís, Maranhão, Brazil., Santos DM; Programas de Pós-Graduação, Universidade Ceuma, São Luís, Maranhão, Brazil., Maia HS; Programas de Pós-Graduação, Universidade Ceuma, São Luís, Maranhão, Brazil., Dos Santos Silva L; Programas de Pós-Graduação, Universidade Ceuma, São Luís, Maranhão, Brazil., da Silva Cutrim B; Programas de Pós-Graduação, Universidade Ceuma, São Luís, Maranhão, Brazil., Vieira SL; Programas de Pós-Graduação, Universidade Ceuma, São Luís, Maranhão, Brazil., Bezerra Filho CM; Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., de Sousa EM; Programas de Pós-Graduação, Universidade Ceuma, São Luís, Maranhão, Brazil., Napoleão TH; Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., Krogfelt KA; Department of Viral and Microbial Diagnostics, Statens Serum Institut, Copenhagen, Denmark.; Department of Science and Environment, Roskilde University, 4000, Roskilde, Denmark., Løbner-Olesen A; Department of Biology, Section for Functional Genomics, University of Copenhagen, Copenhagen, Denmark., Paiva PMG; Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Pernambuco, Brazil., Nascimento da Silva LC; Programas de Pós-Graduação, Universidade Ceuma, São Luís, Maranhão, Brazil. luiscn.silva@ceuma.br.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2019 Dec 03; Vol. 9 (1), pp. 18159. Date of Electronic Publication: 2019 Dec 03.
DOI: 10.1038/s41598-019-54616-x
Abstrakt: Staphylococcus aureus is recognized as an important pathogen causing a wide spectrum of diseases. Here we examined the antimicrobial effects of the lectin isolated from leaves of Schinus terebinthifolia Raddi (SteLL) against S. aureus using in vitro assays and an infection model based on Galleria mellonella larvae. The actions of SteLL on mice macrophages and S. aureus-infected macrophages were also evaluated. SteLL at 16 µg/mL (8 × MIC) increased cell mass and DNA content of S. aureus in relation to untreated bacteria, suggesting that SteLL impairs cell division. Unlike ciprofloxacin, SteLL did not induce the expression of recA, crucial for DNA repair through SOS response. The antimicrobial action of SteLL was partially inhibited by 50 mM N-acetylglucosamine. SteLL reduced staphyloxathin production and increased ciprofloxacin activity towards S. aureus. This lectin also improved the survival of G. mellonella larvae infected with S. aureus. Furthermore, SteLL induced the release of cytokines (IL-6, IL-10, IL-17A, and TNF-α), nitric oxide and superoxide anion by macrophagens. The lectin improved the bactericidal action of macrophages towards S. aureus; while the expression of IL-17A and IFN-γ was downregulated in infected macrophages. These evidences suggest SteLL as important lead molecule in the development of anti-infective agents against S. aureus.
Databáze: MEDLINE
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