In vitro acaricidal effect of Melia azedarach and Artemisia herba - alba extracts on Hyalomma dromedarii (Acari: Ixodidae): embryonated eggs and engorged nymphs.

Autor: Abdel-Ghany HSM; 1Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, Dokki, Giza, Egypt., Fahmy MM; 2Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt., Abuowarda MM; 2Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt., Abdel-Shafy S; 1Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, Dokki, Giza, Egypt., El-Khateeb RM; 1Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, Dokki, Giza, Egypt., Hoballah EM; 3Department of Agriculture Microbiology, Agriculture and Biology Research Division, National Research Centre, Dokki, Giza, Egypt.
Jazyk: angličtina
Zdroj: Journal of parasitic diseases : official organ of the Indian Society for Parasitology [J Parasit Dis] 2019 Dec; Vol. 43 (4), pp. 696-710. Date of Electronic Publication: 2019 Aug 13.
DOI: 10.1007/s12639-019-01149-9
Abstrakt: The present study aimed to evaluate the in vitro efficacy of four medicinal plant extracts: petroleum ether and ethyl alcohol extracts of the ripen fruits of Melia azedarach and whole aerial parts of Artemisia herba - alba against the two inactive stages of the camel tick Hyalomma dromedarii , embryonated eggs and engorged nymphs in comparison to reference acaricide Butox ® 5.0 (Deltamethrin). Egg and nymphal immersion tests at four concentrations with three replicates were used. The deformity in larvae hatched from treated eggs and adults moulted from treated nymphs were observed and photographed by light microscope (LM) and scanning electron microscope (SEM). The results showed that M. azedarach and A. herba - alba extracts revealed higher significant toxic effects on embryonated eggs and engorged nymphs comparing with the reference acaricide (Butox ® 5.0) and control. In egg emmersion test, the LC 50 of petroleum ether extracts of M. azedarach and A. herba - alba was 3.14 and 3.91%, respectively and LC 50 of the respective ethyl alcohol extracts was 1.77 and 2.45%. In nymphal immersion test, LC 50 of petroleum ether extracts of M. azedarach and A. herba - alba was 0.26 and 1%, respectively, and LC 50 of the respective ethyl alcohol extracts was 4.17 and 8.7%. Abnormalities were observed by LM and SEM in the larvae hatched from the treated eggs as incomplete development of legs and mouth parts as well as shrinkage mainly in legs and mouthparts of adults emerged from treated nymphs. In conclusion, all extracts and petroleum ether extracts of the two plants have great potential to be developed as a novel acaricidal for controlling eggs and nymphs of H. dromedarii , respectively.
Competing Interests: Conflict of interestThe authors declare that there is no conflict of interest.
(© Indian Society for Parasitology 2019.)
Databáze: MEDLINE
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