Identification and characterization of the GmRD26 soybean promoter in response to abiotic stresses: potential tool for biotechnological application.

Autor: Freitas EO; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; Federal University of Brasília, Brasília, DF, Brazil., Melo BP; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; Federal University of Viçosa, Viçosa, MG, Brazil., Lourenço-Tessutti IT; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil., Arraes FBM; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil., Amorim RM; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil., Lisei-de-Sá ME; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; Agricultural Research Company of Minas Gerais State, Uberaba, MG, Brazil., Costa JA; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; Catholic University of Brasilia - Post-Graduation Program in Genomic Sciences and Biotechnology, Brasília, DF, Brazil., Leite AGB; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; Federal University of Brasília, Brasília, DF, Brazil., Faheem M; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; National University of Medical Sciences, Rawalpindi, Punjab, Pakistan., Ferreira MA; Federal University of Rio de Janeiro, Rio Janeiro, RJ, Brazil., Morgante CV; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.; Embrapa Semi-Arid, Petrolina, PE, Brazil., Fontes EPB; Federal University of Viçosa, Viçosa, MG, Brazil., Grossi-de-Sa MF; Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil. mariafatimagrossidesa@gmail.com.; Catholic University of Brasilia - Post-Graduation Program in Genomic Sciences and Biotechnology, Brasília, DF, Brazil. mariafatimagrossidesa@gmail.com.
Jazyk: angličtina
Zdroj: BMC biotechnology [BMC Biotechnol] 2019 Nov 20; Vol. 19 (1), pp. 79. Date of Electronic Publication: 2019 Nov 20.
DOI: 10.1186/s12896-019-0561-3
Abstrakt: Background: Drought is one of the most harmful abiotic stresses for plants, leading to reduced productivity of several economically important crops and, consequently, considerable losses in the agricultural sector. When plants are exposed to stressful conditions, such as drought and high salinity, they modulate the expression of genes that lead to developmental, biochemical, and physiological changes, which help to overcome the deleterious effects of adverse circumstances. Thus, the search for new specific gene promoter sequences has proved to be a powerful biotechnological strategy to control the expression of key genes involved in water deprivation or multiple stress responses.
Results: This study aimed to identify and characterize the GmRD26 promoter (pGmRD26), which is involved in the regulation of plant responses to drought stress. The expression profile of the GmRD26 gene was investigated by qRT-PCR under normal and stress conditions in Williams 82, BR16 and Embrapa48 soybean-cultivars. Our data confirm that GmRD26 is induced under water deficit with different induction folds between analyzed cultivars, which display different genetic background and physiological behaviour under drought. The characterization of the GmRD26 promoter was performed under simulated stress conditions with abscisic acid (ABA), polyethylene glycol (PEG) and drought (air dry) on A. thaliana plants containing the complete construct of pGmRD26::GUS (2.054 bp) and two promoter modules, pGmRD26A::GUS (909 pb) and pGmRD26B::GUS (435 bp), controlling the expression of the β-glucuronidase (uidA) gene. Analysis of GUS activity has demonstrated that pGmRD26 and pGmRD26A induce strong reporter gene expression, as the pAtRD29 positive control promoter under ABA and PEG treatment.
Conclusions: The full-length promoter pGmRD26 and the pGmRD26A module provides an improved uidA transcription capacity when compared with the other promoter module, especially in response to polyethylene glycol and drought treatments. These data indicate that pGmRD26A may become a promising biotechnological asset with potential use in the development of modified drought-tolerant plants or other plants designed for stress responses.
Databáze: MEDLINE
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