Xylooligosaccharides production from wheat middlings bioprocessed with Bacillus subtilis.

Autor: Reque PM; Laboratório de Bioquímica e Microbiologia Aplicada, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 91501-970 Porto Alegre, Brazil. Electronic address: priscilla.reque@ufrgs.br., Pinilla CMB; Laboratório de Bioquímica e Microbiologia Aplicada, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 91501-970 Porto Alegre, Brazil., Gautério GV; Laboratório de Microbiologia e Biosseparações, Escola de Química e Alimentos, Universidade Federal do Rio Grande, Av. Itália Km 08, 96201-900 Rio Grande, Brazil., Kalil SJ; Laboratório de Microbiologia e Biosseparações, Escola de Química e Alimentos, Universidade Federal do Rio Grande, Av. Itália Km 08, 96201-900 Rio Grande, Brazil., Brandelli A; Laboratório de Bioquímica e Microbiologia Aplicada, Instituto de Ciência e Tecnologia de Alimentos, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves 9500, 91501-970 Porto Alegre, Brazil.
Jazyk: angličtina
Zdroj: Food research international (Ottawa, Ont.) [Food Res Int] 2019 Dec; Vol. 126, pp. 108673. Date of Electronic Publication: 2019 Sep 10.
DOI: 10.1016/j.foodres.2019.108673
Abstrakt: Prebiotic compounds are substrates selectively metabolized by beneficial gut microbiota causing a health-promoting effect. Despite some prebiotic carbohydrates have been largely studied, xylooligosaccharides (XOS) are important prebiotics derived from arabinoxylans, which are polysaccharides found in cereals. This study aimed to investigate the production of xylanolytic enzymes and XOS during bioprocessing of wheat middlings, a product derived from wheat flour production, using a probiotic Bacillus subtilis. The composition of XOS and the enzymatic and prebiotic activities of resulting B. subtilis cultures were evaluated. The activity of xylanolytic enzymes continuously enhanced during the 72 h bacterial growth, where β-xylosidase presented the highest value (70.31 U/mL). XOS profile and concentration varied considerably between control and bioprocessed samples and among these at different times. Maximum prebiotic activity score was found for the 24 h and 72 h bioprocessed samples (1.73 and 1.61, respectively) using the commercial probiotic Lactobacillus acidophilus LA-5. Wheat middlings showed to be a promising substrate for production of prebiotics like XOS and B. subtilis FTC01 appears to be a good source of xylanolytic enzymes.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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