A comprehensive genome-scale model for Rhodosporidium toruloides IFO0880 accounting for functional genomics and phenotypic data.

Autor:	Dinh HV; Department of Chemical Engineering, The Pennsylvania State University, University Park, 306 Chemical and Biomedical Engineering Building, PA, 16802-4400, USA., Suthers PF; Department of Chemical Engineering, The Pennsylvania State University, University Park, 306 Chemical and Biomedical Engineering Building, PA, 16802-4400, USA., Chan SHJ; Department of Chemical Engineering, The Pennsylvania State University, University Park, 306 Chemical and Biomedical Engineering Building, PA, 16802-4400, USA., Shen Y; Department of Chemistry, Princeton University, 285 Frick Laboratory, Princeton, NJ, 08544, USA.; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, 08540, USA., Xiao T; Department of Chemistry, Princeton University, 285 Frick Laboratory, Princeton, NJ, 08544, USA.; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, 08540, USA., Deewan A; Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champagne, 114 Roger Adams Laboratory MC 712, Urbana, IL, 61801, USA., Jagtap SS; Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champagne, 114 Roger Adams Laboratory MC 712, Urbana, IL, 61801, USA., Zhao H; Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champagne, 114 Roger Adams Laboratory MC 712, Urbana, IL, 61801, USA.; Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA., Rao CV; Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champagne, 114 Roger Adams Laboratory MC 712, Urbana, IL, 61801, USA.; Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA., Rabinowitz JD; Department of Chemistry, Princeton University, 285 Frick Laboratory, Princeton, NJ, 08544, USA.; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, 08540, USA., Maranas CD; Department of Chemical Engineering, The Pennsylvania State University, University Park, 306 Chemical and Biomedical Engineering Building, PA, 16802-4400, USA.
Jazyk:	angličtina
Zdroj:	Metabolic engineering communications [Metab Eng Commun] 2019 Aug 28; Vol. 9, pp. e00101. Date of Electronic Publication: 2019 Aug 28 (Print Publication: 2019).
DOI:	10.1016/j.mec.2019.e00101
Abstrakt:	Rhodosporidium toruloides is a red, basidiomycetes yeast that can accumulate a large amount of lipids and produce carotenoids. To better assess this non-model yeast's metabolic capabilities, we reconstructed a genome-scale model of R. toruloides IFO0880's metabolic network ( iRhto 1108) accounting for 2204 reactions, 1985 metabolites and 1108 genes. In this work, we integrated and supplemented the current knowledge with in-house generated biomass composition and experimental measurements pertaining to the organism's metabolic capabilities. Predictions of genotype-phenotype relations were improved through manual curation of gene-protein-reaction rules for 543 reactions leading to correct recapitulations of 84.5% of gene essentiality data (sensitivity of 94.3% and specificity of 53.8%). Organism-specific macromolecular composition and ATP maintenance requirements were experimentally measured for two separate growth conditions: (i) carbon and (ii) nitrogen limitations. Overall, iRhto 1108 reproduced R. toruloides 's utilization capabilities for 18 alternate substrates, matched measured wild-type growth yield, and recapitulated the viability of 772 out of 819 deletion mutants. As a demonstration to the model's fidelity in guiding engineering interventions, the OptForce procedure was applied on iRhto 1108 for triacylglycerol overproduction. Suggested interventions recapitulated many of the previous successful implementations of genetic modifications and put forth a few new ones. (© 2019 The Authors.)
Databáze:	MEDLINE
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