Performance of CDC Trioplex qPCR during a dengue outbreak in Brazil.

Autor: Colombo TE; Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil; Universidade Paulista (UNIP), São José do Rio Preto, SP, Brazil., Versiani AF; Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil., Dutra KR; Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil., Rubiato JGD; Universidade Paulista (UNIP), São José do Rio Preto, SP, Brazil., Galvão TM; Universidade Paulista (UNIP), São José do Rio Preto, SP, Brazil., Negri Reis AF; Prefeitura de São José do Rio Preto, Departamento de Vigilância em Saúde, São José do Rio Preto, SP, Brazil., Nogueira ML; Faculdade de Medicina de São José do Rio Preto (FAMERP), São José do Rio Preto, SP, Brazil. Electronic address: mauricio.nogueira@edu.famerp.br.
Jazyk: angličtina
Zdroj: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology [J Clin Virol] 2019 Dec; Vol. 121, pp. 104208. Date of Electronic Publication: 2019 Nov 04.
DOI: 10.1016/j.jcv.2019.104208
Abstrakt: Background: In recent years real‑time reverse transcription polymerase chain reaction (real-time RT-PCR) has become a leading technique for nucleic acid detection and quantification of flaviviruses, including Dengue virus (DENV). Trioplex real-time RT-PCR has the advantages of providing the concurrent detection of Zika virus (ZIKV), DENV, and Chikungunya virus (CHIKV) RNA in human serum.
Objective: This study sought to compare the sensitivity and specificity of the Trioplex real-time RT-PCR assay to those provided by CDC DENV TaqMan® RT-qPCR assay and conventional PCR when used for DENV detection in the context of a dengue epidemic.
Study Design: We analyzed 1656 serum samples from symptomatic patients with acute febrile disease for 5 days less between December 2018 and May 2019. The samples were tested using the various PCR-based assays.
Results: Of the 1656 serum samples analyzed, 713 (43%) were laboratory-confirmed as arboviruses: 99.86% (712/713) were confirmed as DENV and 0.14% (1/713) were confirmed as ZIKV. Next, 590 samples were selected, and of these, 331 samples (56.1%) were determined to be positive (Ct < 38) and 259 samples (43.9%) were determined to be negative (Ct > 38) using the Trioplex real-time RT-PCR assay. The multiplex method found that the test exhibits 95% sensitivity and 100% specificity.
Conclusion: This evaluation demonstrates the capacity of the Trioplex real-time RT-PCR assay to detect DENV at a high sensitivity and specificity in a geographic area with a current dengue outbreak and a lower co-circulation of other arboviruses - such as ZIKV and CHIKV, and the results prove it´s applicability as clinical screening test that can serve as a confirmatory test.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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