Stability of a monovalent rotavirus vaccine after exposure to different temperatures observed in KwaZulu-Natal, South Africa.
| Autor: | Asowata OE; Department of Medical Microbiology, Nelson R Mandela School of Medicine, University of KwaZulu-Natal., Ashiru OT; Department of Medical Microbiology, Nelson R Mandela School of Medicine, University of KwaZulu-Natal., Sturm AW; Department of Medical Microbiology, Nelson R Mandela School of Medicine, University of KwaZulu-Natal., Moodley P; Department of Medical Microbiology, Nelson R Mandela School of Medicine, University of KwaZulu-Natal. |
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| Jazyk: | angličtina |
| Zdroj: | African health sciences [Afr Health Sci] 2019 Jun; Vol. 19 (2), pp. 1993-1999. |
| DOI: | 10.4314/ahs.v19i2.22 |
| Abstrakt: | Background: Rotavirus infection and its associated hospitalization of children less than 5 years old in middle- and low-income countries remains a public health challenge. We hypothesized that the Rotarix®potency is affected by non-optimal temperatures which translates into reduced vaccine effectiveness in these settings. Objective: To assess the effect of non-optimal temperatures on the potency of the Rotarix® vaccine in South Africa. Methods: Rotarix® vaccine was exposed to temperatures reflecting breaches in the cold chain. Vero cells (ATCC CCL-81) grown in a 24-well tissue culture plates were infected with Rotarix® vaccine viruses after exposure to non-optimal temperatures and the potency of the vaccine was determined using the plaque assay. Results: Exposure of the Rotarix® vaccine to seasonal temperatures in KwaZulu-Natal for 6 hours and to extreme temperatures of 40oC for 72 hours as well as to -20°C and -80°C for 12 hours did not affect the potency of the vaccine beyond its expected standard of >7 x 10 5 PFU/ml. Conclusion: This study revealed that the Rotarix® vaccine remains potent even after exposure to non-optimal temperatures. However, this study only explored the effect of a constant 'adverse' temperature on vaccine potency and not the effect of temperature fluctuations. (© 2019 Asowata et al.) |
| Databáze: | MEDLINE |
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