Escherichia coli limits Salmonella Typhimurium infections after diet shifts and fat-mediated microbiota perturbation in mice.

Autor: Wotzka SY; Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland., Kreuzer M; Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland., Maier L; European Molecular Biology Laboratory, Heidelberg, Heidelberg, Germany., Arnoldini M; Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland., Nguyen BD; Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland., Brachmann AO; Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland., Berthold DL; Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland., Zünd M; Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland., Hausmann A; Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland., Bakkeren E; Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland., Hoces D; Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland., Gül E; Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland., Beutler M; Max von Pettenkofer Institute, Faculty of Medicine, LMU Munich, Munich, Germany., Dolowschiak T; Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland., Zimmermann M; Institute of Molecular Systems Biology, D-BIOL, ETH Zürich, Zürich, Switzerland., Fuhrer T; Institute of Molecular Systems Biology, D-BIOL, ETH Zürich, Zürich, Switzerland., Moor K; Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland., Sauer U; Institute of Molecular Systems Biology, D-BIOL, ETH Zürich, Zürich, Switzerland., Typas A; European Molecular Biology Laboratory, Heidelberg, Heidelberg, Germany., Piel J; Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland., Diard M; Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland., Macpherson AJ; Maurice Müller Laboratories, University Clinic for Visceral Surgery and Medicine, University of Bern, Bern, Switzerland., Stecher B; Max von Pettenkofer Institute, Faculty of Medicine, LMU Munich, Munich, Germany.; German Center for Infection Research (DZIF), Munich, Germany., Sunagawa S; Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland., Slack E; Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland., Hardt WD; Institute of Microbiology, D-BIOL, ETH Zürich, Zürich, Switzerland. hardt@micro.biol.ethz.ch.
Jazyk: angličtina
Zdroj: Nature microbiology [Nat Microbiol] 2019 Dec; Vol. 4 (12), pp. 2164-2174. Date of Electronic Publication: 2019 Oct 07.
DOI: 10.1038/s41564-019-0568-5
Abstrakt: The microbiota confers colonization resistance, which blocks Salmonella gut colonization 1 . As diet affects microbiota composition, we studied whether food composition shifts enhance susceptibility to infection. Shifting mice to diets with reduced fibre or elevated fat content for 24 h boosted Salmonella Typhimurium or Escherichia coli gut colonization and plasmid transfer. Here, we studied the effect of dietary fat. Colonization resistance was restored within 48 h of return to maintenance diet. Salmonella gut colonization was also boosted by two oral doses of oleic acid or bile salts. These pathogen blooms required Salmonella's AcrAB/TolC-dependent bile resistance. Our data indicate that fat-elicited bile promoted Salmonella gut colonization. Both E. coli and Salmonella show much higher bile resistance than the microbiota. Correspondingly, competitive E. coli can be protective in the fat-challenged gut. Diet shifts and fat-elicited bile promote S. Typhimurium gut infections in mice lacking E. coli in their microbiota. This mouse model may be useful for studying pathogen-microbiota-host interactions, the protective effect of E. coli, to analyse the spread of resistance plasmids and assess the impact of food components on the infection process.
Databáze: MEDLINE
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