Serum Small Extracellular Vesicles Proteome of Tuberculosis Patients Demonstrated Deregulated Immune Response.

Autor: Arya R; Translational Health Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India.; School of Life Sciences, Sambalpur University, Sambalpur, Odisha, 768019, India., Dabral D; Translational Health Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India.; Molecular Physiology, and Molecular Medicine Research Group, School of Medicine, Western Sydney University, Campbelltown Campus, Campbelltown, NSW 2560, Australia., Faruquee HM; Translational Health Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India.; Department of Biotechnology & Genetic Engineering, Islamic University, Kushtia, 7003, Bangladesh., Mazumdar H; Multidisciplinary Research Unit (ICMR), Assam Medical College, Dibrugarh, Assam, 786002, India., Patgiri SJ; Multidisciplinary Research Unit (ICMR), Assam Medical College, Dibrugarh, Assam, 786002, India.; Regional Medical Research Centre, N.E. Region (ICMR), Post Box No.105, Dibrugarh, Assam, 786 001, India., Deka T; Department of Microbiology, Assam Medical College, Dibrugarh, Assam, 786002, India., Basumatary R; Department of Microbiology, Assam Medical College, Dibrugarh, Assam, 786002, India., Kupa RU; Healthcare Laboratory and Research Centre, Nagaland Hospital Authority Kohima, Kohima, Nagaland, 797001, India., Semy C; Healthcare Laboratory and Research Centre, Nagaland Hospital Authority Kohima, Kohima, Nagaland, 797001, India., Kapfo W; Healthcare Laboratory and Research Centre, Nagaland Hospital Authority Kohima, Kohima, Nagaland, 797001, India., Liegise K; Healthcare Laboratory and Research Centre, Nagaland Hospital Authority Kohima, Kohima, Nagaland, 797001, India., Kaur I; Malaria Biology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India., Choedon T; Transcriptional Regulation Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India., Kumar P; Cellular Immunology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India., Behera RK; School of Life Sciences, Sambalpur University, Sambalpur, Odisha, 768019, India., Deori P; Department of TB and Chest Disease, Assam Medical College, Dibrugarh, Assam, 786002, India., Nath R; Department of Microbiology, Assam Medical College, Dibrugarh, Assam, 786002, India., Khalo K; Department of Microbiology, Nagaland Hospital Authority Kohima, Kohima, Nagaland, 797001, India., Saikia L; Multidisciplinary Research Unit (ICMR), Assam Medical College, Dibrugarh, Assam, 786002, India.; Department of Microbiology, Assam Medical College, Dibrugarh, Assam, 786002, India.; Department of Microbiology, Gauhati Medical College, Guwahati, Assam, 781032, India., Khamo V; Healthcare Laboratory and Research Centre, Nagaland Hospital Authority Kohima, Kohima, Nagaland, 797001, India., Nanda RK; Translational Health Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067, India.
Jazyk: angličtina
Zdroj: Proteomics. Clinical applications [Proteomics Clin Appl] 2020 Jan; Vol. 14 (1), pp. e1900062. Date of Electronic Publication: 2019 Oct 09.
DOI: 10.1002/prca.201900062
Abstrakt: Purpose: Detailed understanding of host pathogen interaction in tuberculosis is an important avenue for identifying novel therapeutic targets. Small extracellular vesicles (EVs) like exosomes that are rich in proteins, nucleic acids and lipids, act as messengers and may show altered composition in disease conditions.
Experimental Design: In this case control study, small EVs are isolated from serum of 58 subjects (all male, 33 (15-70) in years) including drug naïve active tuberculosis (ATB: n = 22), non-tuberculosis (NTB: n = 18), and healthy subjects (n = 18). Serum small EVs proteome analysis is carried out using isobaric tag for relative and absolute quantification (iTRAQ) experiments and an independent sample (n = 36) is used for validation.
Results: A set of 132 and 68 proteins are identified in iTRAQ-I (ATB/Healthy) and iTRAQ-II (ATB/NTB) experiments, respectively. Four proteins (KYAT3, SERPINA1, HP, and APOC3) show deregulation (log 2 -fold change > ±0.48, p < 0.05) in ATB with respect to healthy controls and Western blot data corroborated mass spectrometry findings.
Conclusions and Clinical Relevance: These important proteins, involved in neutrophil degranulation, plasma heme scavenging, kynurenine, and lipid metabolism, show deregulation in ATB patients. Identification of such a protein panel in circulating small EVs besides providing novel insights into their role in tuberculosis may prove to be useful targets to develop host-directed therapeutic intervention.
(© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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