Development and validation of a pen side test for Rift Valley fever.

Autor: Cêtre-Sossah C; ASTRE, Univ Montpellier, CIRAD, INRA, Montpellier, France.; CIRAD, UMR ASTRE, Sainte-Clotilde, La Réunion, France., Pédarrieu A; ASTRE, Univ Montpellier, CIRAD, INRA, Montpellier, France., Juremalm M; Boehringer Ingelheim Svanova, Virdingsallé, Uppsala, Sweden., Jansen Van Vuren P; Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, Sandringham-Johannesburg, South Africa., Brun A; INIA-CISA, Valdeolmos, Madrid, Spain., Ould El Mamy AB; ONARDEL, service de pathologies infectieuses, Nouakchott, Mauritania., Héraud JM; Virology Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar., Filippone C; Virology Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar., Ravalohery JP; Virology Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar., Chaabihi H; Agate bioservices, Bagard, France., Albina E; ASTRE, Univ Montpellier, CIRAD, INRA, Montpellier, France.; CIRAD, UMR ASTRE, Petit Bourg, Guadeloupe, France., Dommergues L; CoopADEM - GDS Mayotte, Coconi, Mayotte, France., Paweska J; Centre for Emerging Zoonotic and Parasitic Diseases, National Institute for Communicable Diseases, Sandringham-Johannesburg, South Africa., Cardinale E; ASTRE, Univ Montpellier, CIRAD, INRA, Montpellier, France.; CIRAD, UMR ASTRE, Sainte-Clotilde, La Réunion, France.
Jazyk: angličtina
Zdroj: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2019 Sep 11; Vol. 13 (9), pp. e0007700. Date of Electronic Publication: 2019 Sep 11 (Print Publication: 2019).
DOI: 10.1371/journal.pntd.0007700
Abstrakt: Background: Rift Valley fever (RVF) is one of the main vector borne zoonotic diseases that affects a wide range of ruminants and human beings in Africa and the Arabian Peninsula. A rapid and specific test for RVF diagnosis at the site of a suspected outbreak is crucial for the implementation of control measures.
Methodology/principal Findings: A first-line lateral flow immunochromatographic strip test (LFT) was developed for the detection of the nucleoprotein (N) of the RVF virus (RVFV). Its diagnostic performance characteristics were evaluated using reference stocks isolates recovered from different hosts and in geographic regions mimicking clinical specimens and from known RVF negative serum samples. A high level of diagnostic accuracy (DSe (35/35), DSp (167/169)) was observed, including the absence of cross-reactivity with viruses belonging to different genera.
Conclusion/significance: The fact no specialized reagents and laboratory equipment are needed, make this assay a valuable, first-line diagnostic tool in resource-poor diagnostic territories for on-site RVFV detection, however the staff require training.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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