Antibiotics Drive Microbial Imbalance and Vitiligo Development in Mice.

Autor:	Dellacecca ER; Oncology Research Institute, Loyola University, Chicago, Illinois, USA; Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, Illinois, USA; Department of Dermatology, Northwestern University, Chicago, Illinois, USA., Cosgrove C; Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, Illinois, USA; Department of Dermatology, Northwestern University, Chicago, Illinois, USA., Mukhatayev Z; Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, Illinois, USA; Department of Dermatology, Northwestern University, Chicago, Illinois, USA; Department of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty, Kazakhstan., Akhtar S; Oncology Research Institute, Loyola University, Chicago, Illinois, USA; Department of Surgery, Loyola University, Chicago, Illinois, USA., Engelhard VH; Carter Immunology Center and Department of Microbiology, Immunology, and Cancer Biology, University of Virginia School of Medicine, Charlottesville, Virginia, USA., Rademaker AW; Department of Preventive Medicine, Northwestern University, Chicago, Illinois, USA., Knight KL; Department of Microbiology and Immunology, Loyola University, Chicago,Illinois, USA., Le Poole IC; Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, Illinois, USA; Department of Dermatology, Northwestern University, Chicago, Illinois, USA; Department of Microbiology and Immunology, Northwestern University, Chicago, Illinois, USA. Electronic address: caroline.lepoole@northwestern.edu.
Jazyk:	angličtina
Zdroj:	The Journal of investigative dermatology [J Invest Dermatol] 2020 Mar; Vol. 140 (3), pp. 676-687.e6. Date of Electronic Publication: 2019 Aug 28.
DOI:	10.1016/j.jid.2019.08.435
Abstrakt:	Vitiligo is impacted by environmental triggers. We studied the contribution of the microbiome in FH mice, in which depigmentation is mediated by tyrosinase-reactive T cells. The mice received oral antibiotics and were monitored for depigmentation. The microbiome was studied in fecal and skin samples using 16S rRNA analysis. The resulting T-cell distributions were evaluated. In untreated mice, pigment loss did not expand to the pelage, whereas mice in the ampicillin group were approximately 1/3 depigmented at 30 weeks. In contrast to models of autoimmunity that are less dependent on IFN-γ, ampicillin but not neomycin treatment correlated with accelerated disease and reduced bacteria in the fecal pellets. Modified cytokine patterns in the tissue and serum suggest a response that transcends the gut. Ampicillin-induced depigmentation was accompanied by gut but not skin dysbiosis, and reduced T cell numbers in both sites. Neomycin induced a redistribution of gut T cells and an accumulation of skin regulatory T cells. This treatment spurred a Bacteroides-dominated population of fecal bacteria. Reduced diversity is prominent particularly after ampicillin treatment, when the gut is dominated by Pseudomonas species. In line with current concepts relating the microbiome and the immune system, we predict that dietary measures might promote skin health and delay vitiligo onset. (Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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