Dominant inhibition of awn development by a putative zinc-finger transcriptional repressor expressed at the B1 locus in wheat.

Autor: Huang D; Aquatic and Crop Resource Development, National Research Council of Canada, Saskatoon, SK, S7N 0W9, Canada., Zheng Q; Aquatic and Crop Resource Development, National Research Council of Canada, Saskatoon, SK, S7N 0W9, Canada., Melchkart T; Aquatic and Crop Resource Development, National Research Council of Canada, Saskatoon, SK, S7N 0W9, Canada., Bekkaoui Y; Aquatic and Crop Resource Development, National Research Council of Canada, Saskatoon, SK, S7N 0W9, Canada., Konkin DJF; Aquatic and Crop Resource Development, National Research Council of Canada, Saskatoon, SK, S7N 0W9, Canada., Kagale S; Aquatic and Crop Resource Development, National Research Council of Canada, Saskatoon, SK, S7N 0W9, Canada., Martucci M; Morden Research and Development Centre, Agriculture and Agri-Food Canada, 101 Route 100, Morden, MB, R6M 1Y5, Canada., You FM; Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, 960 Carling Avenue, Ottawa, ON, K1A 0C6, Canada., Clarke M; Department of Crop Genetics, John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK., Adamski NM; Department of Crop Genetics, John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK., Chinoy C; Department of Crop Genetics, John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK., Steed A; Department of Crop Genetics, John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK., McCartney CA; Morden Research and Development Centre, Agriculture and Agri-Food Canada, 101 Route 100, Morden, MB, R6M 1Y5, Canada., Cutler AJ; Aquatic and Crop Resource Development, National Research Council of Canada, Saskatoon, SK, S7N 0W9, Canada., Nicholson P; Department of Crop Genetics, John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK., Feurtado JA; Aquatic and Crop Resource Development, National Research Council of Canada, Saskatoon, SK, S7N 0W9, Canada.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2020 Jan; Vol. 225 (1), pp. 340-355. Date of Electronic Publication: 2019 Oct 10.
DOI: 10.1111/nph.16154
Abstrakt: Awns, bristle-like structures extending from grass lemmas, provide protection against predators, contribute to photosynthesis and aid in grain dispersal. In wheat, selection of awns with minimal extension, termed awnletted, has occurred during domestication by way of loci that dominantly inhibit awn development, such as Tipped1 (B1), Tipped2 (B2), and Hooded (Hd). Here we identify and characterize the B1 gene. B1 was identified using bulked segregant RNA-sequencing of an F 2 durum wheat population and through deletion mapping of awned bread wheat mutants. Functional characterization was accomplished by gene overexpression while haplotype analyses assessed B1 polymorphisms and genetic variation. Located on chromosome 5A, B1 is a C2H2 zinc finger encoding gene with ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motifs. Constitutive overexpression of B1 in awned wheat produced an awnletted phenotype with pleiotropic effects on plant height and fertility. Transcriptome analysis of B1 overexpression plants suggests a role as transcriptional repressor, putatively targeting pathways involved in cell proliferation. Haplotype analysis revealed a conserved B1 coding region with proximal polymorphisms and supported the contention that B1 is mainly responsible for awnletted wheats globally. B1, predominantly responsible for awn inhibition in wheat, encodes a C2H2 zinc finger protein with EAR motifs which putatively functions as a transcriptional repressor.
(© 2019 National Research Council Canada New Phytologist © 2019 New Phytologist Trust.)
Databáze: MEDLINE
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