Feline coronavirus isolates from a part of Brazil: insights into molecular epidemiology and phylogeny inferred from the 7b gene.

Autor: Myrrha LW; Laboratory of Animal Molecular Infectology, Institute of Biotechnology Applied to Agriculture, Federal University of Viçosa (UFV), Viçosa, Minas Gerais 36570-900, Brazil., Silva FMF; Laboratory of Animal Molecular Infectology, Institute of Biotechnology Applied to Agriculture, Federal University of Viçosa (UFV), Viçosa, Minas Gerais 36570-900, Brazil., Vidigal PMP; Nucleus of Analysis of Biomolecules, Center of Biological Sciences, Federal University of Viçosa (UFV), Viçosa, Minas Gerais 36570-900, Brazil., Resende M; Departament of Microbiology, Federal University of Minas Gerais (UFMG), Belo Horizonte, Minas Gerais 31275-035, Brazil., Bressan GC; Laboratory of Animal Molecular Infectology, Institute of Biotechnology Applied to Agriculture, Federal University of Viçosa (UFV), Viçosa, Minas Gerais 36570-900, Brazil., Fietto JLR; Laboratory of Animal Molecular Infectology, Institute of Biotechnology Applied to Agriculture, Federal University of Viçosa (UFV), Viçosa, Minas Gerais 36570-900, Brazil., Santos MR; Laboratory of Immunobiological and Animal Virology, Departament of Veterinary, Federal University of Viçosa (UFV), Viçosa, Minas Gerais 36570-900, Brazil., Silva LMN; Laboratory of Immunobiological and Animal Virology, Departament of Veterinary, Federal University of Viçosa (UFV), Viçosa, Minas Gerais 36570-900, Brazil., Assao VS; Laboratory of Immunobiological and Animal Virology, Departament of Veterinary, Federal University of Viçosa (UFV), Viçosa, Minas Gerais 36570-900, Brazil., Silva-Jú Nior A; Laboratory of Immunobiological and Animal Virology, Departament of Veterinary, Federal University of Viçosa (UFV), Viçosa, Minas Gerais 36570-900, Brazil., de Almeida MR; Laboratory of Animal Molecular Infectology, Institute of Biotechnology Applied to Agriculture, Federal University of Viçosa (UFV), Viçosa, Minas Gerais 36570-900, Brazil.
Jazyk: angličtina
Zdroj: The Journal of veterinary medical science [J Vet Med Sci] 2019 Oct 18; Vol. 81 (10), pp. 1455-1460. Date of Electronic Publication: 2019 Aug 23.
DOI: 10.1292/jvms.19-0090
Abstrakt: The Feline coronavirus (FCoV) can lead to Feline infectious peritonitis (FIP), which the precise cause is still unknown. The theory of internal mutation suggests that a less virulent biotype of FCoV (FECV) would lead to another more pathogenic biotype (FIPV) capable of causing FIP. In this work, the 7b gene was amplified from 51 domestic cat plasma samples by semi-nested PCR and tested through phylogenetic and phylogeographical approaches. The 7b gene of Brazilian isolates displayed high conservation, a strong correlation between the geographic origin of the viral isolates and their genealogy, and its evolution was possibly shaped by a combination of high rates of nucleotide substitution and purifying selection.
Databáze: MEDLINE