Divergent allocation of sperm and the seminal proteome along a competition gradient in Drosophila melanogaster .

Autor:	Hopkins BR; Edward Grey Institute, Department of Zoology, University of Oxford, OX1 3PS Oxford, United Kingdom; brhopkins92@gmail.com., Sepil I; Edward Grey Institute, Department of Zoology, University of Oxford, OX1 3PS Oxford, United Kingdom., Thézénas ML; TDI Mass Spectrometry Laboratory, Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, OX3 7BN Oxford, United Kingdom., Craig JF; Edward Grey Institute, Department of Zoology, University of Oxford, OX1 3PS Oxford, United Kingdom., Miller T; Edward Grey Institute, Department of Zoology, University of Oxford, OX1 3PS Oxford, United Kingdom., Charles PD; TDI Mass Spectrometry Laboratory, Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, OX3 7BN Oxford, United Kingdom., Fischer R; TDI Mass Spectrometry Laboratory, Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, OX3 7BN Oxford, United Kingdom., Kessler BM; TDI Mass Spectrometry Laboratory, Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, OX3 7BN Oxford, United Kingdom., Bretman A; School of Biology, Faculty of Biological Sciences, University of Leeds, LS2 9JT Leeds, United Kingdom., Pizzari T; Edward Grey Institute, Department of Zoology, University of Oxford, OX1 3PS Oxford, United Kingdom., Wigby S; Edward Grey Institute, Department of Zoology, University of Oxford, OX1 3PS Oxford, United Kingdom.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2019 Sep 03; Vol. 116 (36), pp. 17925-17933. Date of Electronic Publication: 2019 Aug 20.
DOI:	10.1073/pnas.1906149116
Abstrakt:	Sperm competition favors large, costly ejaculates, and theory predicts the evolution of allocation strategies that enable males to plastically tailor ejaculate expenditure to sperm competition threat. While greater sperm transfer in response to a perceived increase in the risk of sperm competition is well-supported, we have a poor understanding of whether males ( i ) respond to changes in perceived intensity of sperm competition, ( ii ) use the same allocation rules for sperm and seminal fluid, and ( iii ) experience changes in current and future reproductive performance as a result of ejaculate compositional changes. Combining quantitative proteomics with fluorescent sperm labeling, we show that Drosophila melanogaster males exercise independent control over the transfer of sperm and seminal fluid proteins (SFPs) under different levels of male-male competition. While sperm transfer peaks at low competition, consistent with some theoretical predictions based on sperm competition intensity, the abundance of transferred SFPs generally increases at high competition levels. However, we find that clusters of SFPs vary in the directionality and sensitivity of their response to competition, promoting compositional change in seminal fluid. By tracking the degree of decline in male mating probability and offspring production across successive matings, we provide evidence that ejaculate compositional change represents an adaptive response to current sperm competition, but one that comes at a cost to future mating performance. Our work reveals a previously unknown divergence in ejaculate component allocation rules, exposes downstream costs of elevated ejaculate investment, and ultimately suggests a central role for ejaculate compositional plasticity in sexual selection. Competing Interests: The authors declare no conflict of interest. (Copyright © 2019 the Author(s). Published by PNAS.)
Databáze:	MEDLINE
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