A seed germination transcriptomic study contrasting two soybean genotypes that differ in terms of their tolerance to the deleterious impacts of elevated temperatures during seed fill.

Autor: Gillman JD; USDA-ARS, Plant Genetics Research Unit, 205 Curtis Hall, University of Missouri, Columbia, MO, 65211, USA. Jason.Gillman@ars.usda.gov., Biever JJ; Divisions of Plant Science, University of Missouri-Columbia, Columbia, MO, 65211, USA.; Metropolitan Community College-Penn Valley, Kansas City, MO, USA., Ye S; Divisions of Plant Science, University of Missouri-Columbia, Columbia, MO, 65211, USA., Spollen WG; Informatics Research Core Facility, University of Missouri-Columbia, Columbia, MO, 65211, USA., Givan SA; Bioinformatics and Biostatistics Core, Van Andel Research Institute, University of Missouri-Columbia, Columbia, MO, USA., Lyu Z; Department of Electrical Engineering and Computer Science, University of Missouri-Columbia, Columbia, MO, USA., Joshi T; Health Management and Informatics, MU Informatics Institute, Interdisciplinary Plant Group and Christopher S. Bond Life Science Center, University of Missouri-Columbia, Columbia, MO, 65211, USA., Smith JR; USDA-ARS, Crop Genetics Research Unit, Stoneville, MS, 38776, USA., Fritschi FB; Divisions of Plant Science, University of Missouri-Columbia, Columbia, MO, 65211, USA.
Jazyk: angličtina
Zdroj: BMC research notes [BMC Res Notes] 2019 Aug 19; Vol. 12 (1), pp. 522. Date of Electronic Publication: 2019 Aug 19.
DOI: 10.1186/s13104-019-4559-7
Abstrakt: Objective: Soybean seed development is negatively impacted by elevated temperatures during seed fill, which can decrease seed quality and economic value. Prior germplasm screens identified an exotic landrace able to maintain ~ 95% seed germination under stress conditions that reduce germination dramatically (> 50%) for typical soybean seeds. Seed transcriptomic analysis was performed for two soybean lines (a heat-tolerant landrace and a typical high-yielding adapted line) for dry, mature seed, 6-h imbibed seed and germinated seed. Seeds were produced in two environments: a typical Midwestern field and a heat stressed field located in the Midsouth soybean production region.
Results: Transcriptomic analysis revealed 23-30K expressed genes in each seed tissue sample, and differentially expressed genes (DEGs) with ≥ twofold gene expression differences (at q-value < 0.05) comprised ~ 5-44% of expressed genes. Gene ontology (GO) enrichment analysis on DEGs revealed enrichment in heat-tolerant seeds for genes annotated for general and temperature-specific stress, as well as protein-refolding. DEGs were also clustered in modules using weighted co-expressed gene network analysis, which were examined for enrichment of GO biological process terms. Collectively, our results provide new and valuable insights into this unique form of genetic abiotic stress tolerance and to soybean seed physiological responses to elevated temperatures.
Databáze: MEDLINE
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