Sweet taste receptor agonists alter ovarian functions and ovarian cycles in aged mice.

Autor: Ngekure M X K; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China., Jiang J; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China., Enayatullah H; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China., Ennab W; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China., Mustafa S; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China., Rodeni S; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China., Wei Q; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China., Shi F; College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: fxshi@njau.edu.cn.
Jazyk: angličtina
Zdroj: Reproductive biology [Reprod Biol] 2019 Sep; Vol. 19 (3), pp. 230-236. Date of Electronic Publication: 2019 Aug 06.
DOI: 10.1016/j.repbio.2019.07.007
Abstrakt: Saccharine sodium and rebaudioside A are low-calorie sweeteners, and the biologic effects of these sweeteners in rat ovaries are related to the activity of sweet taste receptors. Data on the impact and regulatory mechanisms underlying such sweeteners on the reproduction of aged animals are currently lacking. In the present study we assessed how the consumption of sweeteners affects the ovarian cycle, ovulation, biochemical indices, and other biologic functions. Thirty-six 1-year-old mice were randomly divided into 3 groups: a control (C) group receiving regular water, a saccharin sodium group receiving a 7.5 mM solution, and the rebaudioside A group receiving a 2.5 mM solution for 30 days. We observed no significant changes in body weights in any group. However, uterine weight in the rebaudioside A group significantly increased in diestrus, and we recorded a significant increase in the percentage of abnormal estrous cycles and the number of corpora lutea in the treatment groups. TUNEL staining and Immunoreactivity for the apoptosis-inducing factor (AIF) confirmed apoptosis in granulosa cells, oocyte, and corpus luteum. Serum glucose increased significantly in both treatment groups and there was a significant increase in cholesterol in the rebaudioside A group. Furthermore, the saccharin sodium-treated group exhibited elevated serum progesterone levels compared with the other groups. In conclusion, sweeteners manifested deleterious effects on reproductive indices in aged mice.
(Copyright © 2019 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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