Improved efficacy of naproxen-loaded NLC for temporomandibular joint administration.

Autor: Guilherme VA; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Ribeiro LNM; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Alcântara ACS; Department of Chemistry, Federal University of Maranhão - UFMA, São Luis, Maranhão, Brazil., Castro SR; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Rodrigues da Silva GH; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., da Silva CG; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil., Breitkreitz MC; Department of Analytical Chemistry, Institute of Chemistry, UNICAMP, Campinas, São Paulo, Brazil., Clemente-Napimoga J; Department of Physiological Science, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil.; Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Campinas, São Paulo, Brazil., Macedo CG; Department of Physiological Science, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil., Abdalla HB; Department of Physiological Science, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil., Bonfante R; Department of Physiological Science, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil., Cereda CMS; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil.; Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Campinas, São Paulo, Brazil., de Paula E; Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil. depaula@unicamp.br.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2019 Aug 01; Vol. 9 (1), pp. 11160. Date of Electronic Publication: 2019 Aug 01.
DOI: 10.1038/s41598-019-47486-w
Abstrakt: Inflammatory conditions of the temporomandibular joint (TMJ) and peripheral tissues affect many people around the world and are commonly treated with non-steroidal anti-inflammatory drugs (NSAIDs). However, in order to get desirable results, treatments with NSAIDs may take weeks, causing undesirable side effects and requiring repeated administration. In this sense, this work describes the development of an optimized nanostructured lipid carrier (NLC) formulation for intra-articular administration of naproxen (NPX). An experimental design (2 3 ) selected the best formulation in terms of its physicochemical and structural properties, elucidated by different methods (DLS, NTA, TEM, DSC, and ATR-FTIR). The chosen formulation (NLC-NPX) was tested on acute inflammatory TMJ nociception, in a rat model. The optimized excipients composition provided higher NPX encapsulation efficiency (99.8%) and the nanoparticles were found stable during 1 year of storage at 25 °C. In vivo results demonstrated that the sustained delivery of NPX directly in the TMJ significantly reduced leukocytes migration and levels of pro-inflammatory cytokines (IL-1β and TNF-α), for more than a week. These results point out the NLC-NPX formulation as a promising candidate for the safe treatment of inflammatory pain conditions of TMJ or other joints.
Databáze: MEDLINE
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