A biomimetic self-assembling peptide promotes bone regeneration in vivo: A rat cranial defect study.

Autor: Saha S; Department of Oral Biology, School of Dentistry, St James's University Hospital, University of Leeds, Leeds, UK., Yang XB; Department of Oral Biology, School of Dentistry, St James's University Hospital, University of Leeds, Leeds, UK., Wijayathunga N; School of Mechanical Engineering, University of Leeds, Leeds, UK., Harris S; School of Physics and Astronomy, Astbury Centre for Structural and Molecular Biology, University of Leeds, Leeds, UK., Feichtinger GA; Department of Oral Biology, School of Dentistry, St James's University Hospital, University of Leeds, Leeds, UK., Davies RPW; Department of Oral Biology, School of Dentistry, St James's University Hospital, University of Leeds, Leeds, UK. Electronic address: R.P.W.Davies@leeds.ac.uk., Kirkham J; Department of Oral Biology, School of Dentistry, St James's University Hospital, University of Leeds, Leeds, UK.
Jazyk: angličtina
Zdroj: Bone [Bone] 2019 Oct; Vol. 127, pp. 602-611. Date of Electronic Publication: 2019 Jul 24.
DOI: 10.1016/j.bone.2019.06.020
Abstrakt: Rationally designed, pH sensitive self-assembling β-peptides (SAPs) which are capable of reversibly switching between fluid and gel phases in response to environmental triggers are potentially useful injectable scaffolds for skeletal tissue engineering applications. SAP P 11 -4 (CH 3 COQQRFEWEFEQQNH 2 ) has been shown to nucleate hydroxyapatite mineral de novo and has been used in dental enamel regeneration. We hypothesised that addition of mesenchymal stromal cells (MSCs) would enhance the in vivo effects of P 11 -4 in promoting skeletal tissue repair. Cranial defects were created in athymic rats and filled with either Bio-Oss® (anorganic bone chips) or P 11 -4 ± human dental pulp stromal cells (HDPSCs). Unfilled defects served as controls. After 4 weeks, only those defects filled with P 11 -4 alone showed significantly increased bone regeneration (almost complete healing), compared to unfilled control defects, as judged using quantitative micro-CT, histology and immunohistochemistry. In silico modelling indicated that fibril formation may be essential for any mineral nucleation activity. Taken together, these data suggest that self-assembling peptides are a suitable scaffold for regeneration of bone tissue in a one step, cell-free therapeutic approach.
(Crown Copyright © 2019. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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