Engineering Dual Variable Domains for the Generation of Site-Specific Antibody-Drug Conjugates.

Autor:	Nanna AR; Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL, USA.; Department of Chemistry, The Scripps Research Institute, Jupiter, FL, USA., Rader C; Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL, USA. crader@scripps.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 2033, pp. 39-52.
DOI:	10.1007/978-1-4939-9654-4_4
Abstrakt:	Site-specific antibody-drug conjugate (ADC) technologies are highly desirable for the production of therapeutics with well-defined biochemical and pharmacological characteristics. We have developed a strategy to produce site-specific ADCs using a highly reactive lysine residue embedded in a dual-variable-domain (DVD) format. Here we provide protocols for the engineering, expression, and purification of the DVDs used for this strategy. We also provide a protocol for DVD-drug conjugation and describe methods for their biochemical characterization, including a catalytic assay to monitor conjugation efficiency.
Databáze:	MEDLINE
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