Carriage of carbapenemase- and extended-spectrum cephalosporinase-producing Escherichia coli and Klebsiella pneumoniae in humans and livestock in rural Cambodia; gender and age differences and detection of bla OXA-48 in humans.

Autor: Atterby C; Zoonosis Science Center, Department of Medical Sciences, Uppsala University, Uppsala, Sweden., Osbjer K; Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden.; Food and Agriculture Organization of the United Nations, Phnom Penh, Cambodia., Tepper V; Institute of Environmental Engineering, ETH Zürich, Switzerland., Rajala E; Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden., Hernandez J; Center for Ecology and Evolution in Microbial Model Systems Linnaeus University, Kalmar, Sweden.; Department of Infectious Diseases, Kalmar County Council, Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.; Diagnostic Centrum, Clinic Microbiologic Laboratory, Kalmar County Hospital, Kalmar, Sweden., Seng S; Food and Agriculture Organization of the United Nations, Phnom Penh, Cambodia., Holl D; General Directorate of Animal Health and Production, Ministry of Agriculture, Forestry and Fisheries, Phnom Penh, Cambodia., Bonnedahl J; Center for Ecology and Evolution in Microbial Model Systems Linnaeus University, Kalmar, Sweden.; Department of Infectious Diseases, Kalmar County Council, Department of Clinical and Experimental Medicine, Linköping University, Linköping, Sweden., Börjesson S; Department of Animal Health and Antimicrobial strategies, National Veterinary Institute (SVA), Uppsala, Sweden.; Department of Clinical and Experimental Medicine, Linköping University, Sweden., Magnusson U; Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden., Järhult JD; Zoonosis Science Center, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
Jazyk: angličtina
Zdroj: Zoonoses and public health [Zoonoses Public Health] 2019 Sep; Vol. 66 (6), pp. 603-617. Date of Electronic Publication: 2019 Jul 02.
DOI: 10.1111/zph.12612
Abstrakt: Objectives: This study investigates the frequency and characteristics of carbapenemase-producing Escherichia coli/Klebsiella pneumoniae (CPE/K) and extended-spectrum cephalosporinase-producing E. coli/K. pneumoniae (ESCE/K) in healthy humans and livestock in rural Cambodia. Additionally, household practices as risk factors for faecal carriage of ESCE/K are identified.
Methods: Faecal samples were obtained from 307 humans and 285 livestock including large ruminants, pigs and poultry living in 100 households in rural Cambodia in 2011. Each household was interviewed, and multilevel logistic model determined associations between household practices/meat consumption and faecal carriage of ESCE/K. CPE and ESCE/K were detected and further screened for colistin resistance genes.
Results: CPE/K isolates harbouring bla OXA-48 were identified in two humans. The community carriage of ESCE/K was 20% in humans and 23% in livestock. The same ESBL genes: bla CTX-M-15 , bla CTX-M-14 , bla CTX-M-27 , bla CTX-M-55 , bla SHV-2 , bla SHV-12 , bla SHV-28 ; AmpC genes: bla CMY-2 , bla CMY-42, bla DHA-1 ; and colistin resistance genes: mcr-1-like and mcr-3-like were detected in humans and livestock. ESCE/K was frequently detected in women, young children, pigs and poultry, which are groups in close contact. The practice of burning or burying meat waste and not collecting animal manure indoors and outdoors daily were identified as risk factors for faecal carriage of ESCE/K.
Conclusions: Faecal carriage of E. coli and K. pneumoniae harbouring extended-spectrum cephalosporinase genes are common in the Cambodian community, especially in women and young children. Exposure to animal manure and slaughter products are risk factors for intestinal colonization of ESCE/K in humans.
(© 2019 The Authors. Zoonoses and Public Health Published by Blackwell Verlag GmbH.)
Databáze: MEDLINE
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