Compositional analysis and biological characterization of Cornus officinalis on human 1.1B4 pancreatic β cells.

Autor: Sharp-Tawfik AE; Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, 4202 East Fowler Avenue, Tampa, FL 33620, USA., Coiner AM; Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, 4202 East Fowler Avenue, Tampa, FL 33620, USA., MarElia CB; Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, 4202 East Fowler Avenue, Tampa, FL 33620, USA., Kazantzis M; Metabolic Core, The Scripps Research Institute, 130 Scripps Way, Jupiter, FL 33458, USA., Zhang C; Practice of Oriental Medicine, Tucson, AZ, USA., Burkhardt BR; Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, 4202 East Fowler Avenue, Tampa, FL 33620, USA. Electronic address: bburkhardt@usf.edu.
Jazyk: angličtina
Zdroj: Molecular and cellular endocrinology [Mol Cell Endocrinol] 2019 Aug 20; Vol. 494, pp. 110491. Date of Electronic Publication: 2019 Jun 27.
DOI: 10.1016/j.mce.2019.110491
Abstrakt: Type 1 diabetes (T1D) is an autoimmune disease resulting from the loss of pancreatic β cells and subsequent insulin production. Novel interventional therapies are urgently needed that can protect existing β cells from cytokine-induced death and enhance their function before symptomatic onset. Our initial evidence is suggesting that bioactive ingredients within Cornus officinalis (CO) may be able to serve in this function. CO has been extensively used in Traditional Chinese Medicine (TCM) and reported to possess both anti-inflammatory and pro-metabolic effects. We hypothesize that CO treatment may provide a future potential candidate for interventional therapy for early stage T1D prior to significant β cell loss. Our data demonstrated that CO can inhibit cytokine-mediated β cell death, increase cell viability and oxidative capacity, and increase expression of NFATC2 (Nuclear Factor of Activated T Cells, Cytoplasmic 2). We have also profiled the bioactive components in CO from multiple sources by HPLC/MS (High Performance Liquid Chromatography/Mass Spectrometry) analysis. Altogether, CO significantly increases the energy metabolism of β cells while inducing the NFAT pathway to signal for increased proliferation and endocrine function.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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