Circulating microvesicle protein is associated with renal transplant outcome.

Autor: Al-Nedawi K; Division of Nephrology, Department of Medicine, McMaster University, Hamilton, Ontario, Canada; St. Joseph's Healthcare Research Institute & Hamilton Center for Kidney Research, Canada. Electronic address: alnedaw@mcmaster.ca., Haas-Neill S; Division of Nephrology, Department of Medicine, McMaster University, Hamilton, Ontario, Canada; St. Joseph's Healthcare Research Institute & Hamilton Center for Kidney Research, Canada., Gangji A; Division of Nephrology, Department of Medicine, McMaster University, Hamilton, Ontario, Canada; St. Joseph's Healthcare Research Institute & Hamilton Center for Kidney Research, Canada., Ribic CM; Division of Nephrology, Department of Medicine, McMaster University, Hamilton, Ontario, Canada; St. Joseph's Healthcare Research Institute & Hamilton Center for Kidney Research, Canada., Kapoor A; St. Joseph's Healthcare Research Institute & Hamilton Center for Kidney Research, Canada; Department of Surgery, McMaster University, Hamilton, Ontario, Canada., Margetts P; Division of Nephrology, Department of Medicine, McMaster University, Hamilton, Ontario, Canada; St. Joseph's Healthcare Research Institute & Hamilton Center for Kidney Research, Canada.
Jazyk: angličtina
Zdroj: Transplant immunology [Transpl Immunol] 2019 Aug; Vol. 55, pp. 101210. Date of Electronic Publication: 2019 Jun 19.
DOI: 10.1016/j.trim.2019.06.002
Abstrakt: Renal transplantation is an effective therapy with improved long-term outcomes compared with other therapies for end stage renal disease. Present methods for evaluating kidney allograft function, such as serum creatinine or allograft biopsy, are not sensitive and identify pathological changes only after any potential intervention would be effective. Thus, there is a necessity for biomarkers that would provide early prognostic information about kidney transplant outcomes. Circulating microvesicles represent an attractive source of biomarkers for different diseases including renal failure. We have studied the proteins of the circulating microvesicles from two populations of kidney transplant recipients (n = 20) with poor transplant outcomes (n = 10) or good transplant outcome (n = 10), according to their estimated glomerular filtration rate (eGFR). Microvesicles from age-matched healthy subjects (n = 10) were used as a control. Also, we performed a pilot study to assess the microvesicle protein in kidney transplant recipients before and six months after kidney transplant (n = 6), compared to healthy subjects. Proteomic analysis of microvesicles could discriminate between transplant recipients and healthy subjects, and between transplant patients based on eGFR. Our results shed light on the potential of blood microvesicles to provide a novel tool for the prediction of the outcome of kidney transplants.
(Copyright © 2019. Published by Elsevier B.V.)
Databáze: MEDLINE
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