| Autor: |
Falavigna VDS; Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, 91501-970, Brazil.; AGAP, Univ. Montpellier, CIRAD, INRA, Montpellier SupAgro, Montpellier, France., Malabarba J; Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, 91501-970, Brazil., Silveira CP; Embrapa Uva e Vinho, Rua Livramento, 515, P.O. Box 130, Bento Gonçalves, RS, 95701-008, Brazil., Buffon V; Embrapa Uva e Vinho, Rua Livramento, 515, P.O. Box 130, Bento Gonçalves, RS, 95701-008, Brazil., Mariath JEA; Laboratory of Plant Anatomy, Botany Department, UFRGS, Porto Alegre, 91501-970, Brazil., Pasquali G; Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, 91501-970, Brazil., Margis-Pinheiro M; Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, 91501-970, Brazil., Revers LF; Graduate Program in Cell and Molecular Biology, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, 91501-970, Brazil. luis.revers@embrapa.br.; Embrapa Uva e Vinho, Rua Livramento, 515, P.O. Box 130, Bento Gonçalves, RS, 95701-008, Brazil. luis.revers@embrapa.br. |
| Abstrakt: |
Key Message: MdoDHN11 acts in the nucellus layer to protect the embryo and the endosperm from limited water availability during apple seed development. Dehydrins (DHNs) are protective proteins related to several plant developmental responses that involve dehydration such as seed desiccation and abiotic stresses. In apple (Malus × domestica Borkh.), the seed-specific MdoDHN11 was suggested to play important roles against dehydration during seed development. However, this hypothesis has not yet been evaluated. Within this context, several experiments were performed to functionally characterize MdoDHN11. In situ hybridization analysis during apple seed development showed that MdoDHN11 expression is confined to a maternal tissue called nucellus, a central mass of parenchyma between the endosperm and the testa. The MdoDHN11 protein was localized in the cytosol and nucleus. Finally, transgenic Arabidopsis plants expressing MdoDHN11 were generated and exposed to a severe water-deficit stress, aiming to mimic a situation that can occurs during seed development. All transgenic lines showed increased tolerance to water deficit in relation to wild-type plants. Taken together, our results provide evidences that MdoDHN11 plays important roles during apple seed development by protecting the embryo and the endosperm from limited water availability, and the mechanism of action probably involves the interaction of MdoDHN11 with proteins and other components in the cell. |
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