Plasmodium vivax ookinete surface protein (Pvs25) is highly conserved among field isolates from five different regions of the Brazilian Amazon.

Autor: Chaves LB; Laboratório de Imunoparasitologia, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, RJ, Brazil., Perce-da-Silva DS; Laboratório de Pesquisa em Malária, IOC, Fiocruz, Rio de Janeiro, RJ, Brazil., Totino PRR; Laboratório de Pesquisa em Malária, IOC, Fiocruz, Rio de Janeiro, RJ, Brazil., Riccio EKP; Laboratório de Pesquisa em Malária, IOC, Fiocruz, Rio de Janeiro, RJ, Brazil., Baptista BO; Laboratório de Pesquisa em Malária, IOC, Fiocruz, Rio de Janeiro, RJ, Brazil., de Souza ABL; Laboratório de Pesquisa em Malária, IOC, Fiocruz, Rio de Janeiro, RJ, Brazil., Rodrigues-da-Silva RN; Laboratório de Imunoparasitologia, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, RJ, Brazil., Machado RLD; Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Brazil., de Souza RM; Centro de Pesquisa em Doenças Infecciosas, Centro Multidisciplinar, Universidade Federal do Acre, Campus Floresta, Brazil., Daniel-Ribeiro CT; Laboratório de Pesquisa em Malária, IOC, Fiocruz, Rio de Janeiro, RJ, Brazil., Banic DM; Laboratório de Imunologia Clínica, IOC, Fiocruz, Rio de Janeiro, RJ, Brazil., Pratt-Riccio LR; Laboratório de Pesquisa em Malária, IOC, Fiocruz, Rio de Janeiro, RJ, Brazil., Lima-Junior JDC; Laboratório de Imunoparasitologia, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, RJ, Brazil. Electronic address: josue@ioc.fiocruz.br.
Jazyk: angličtina
Zdroj: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2019 Sep; Vol. 73, pp. 287-294. Date of Electronic Publication: 2019 May 08.
DOI: 10.1016/j.meegid.2019.05.003
Abstrakt: The Plasmodium vivax Ookinete Surface Protein (Pvs25) is one of the leading malaria Transmission-Blocking Vaccine candidates based on its high immunogenicity in animal models, transmission-blocking activity of antibodies elicited in clinical trials and high conservation among P. vivax isolates from endemic areas. However, the polymorphism in gene encoding Pvs25 in endemic areas from South America has been poorly studied so far. Here, we investigated the genetic polymorphism of pvs25 in P. vivax isolates from five different regions of the Brazilian Amazon (Cruzeiro do Sul, Mâncio Lima, Guajará, Manaus and Oiapoque) and its impact on antigenicity of predicted B-cell epitopes using gene sequencing and epitope prediction tools. Firstly, only a non-synonymous substitution was found in the 657 bp amplified fragment in all sequenced samples, which represented an exchange of Gln by Lys at position 87 (Q87K) of protein amino acid sequence (domain II EGF-like). Q87K substitution was also present in all studied sites with a total frequency of 37.8%. Cruzeiro do Sul presented Q87K substitution in almost half of the isolates (48.4%), and an expressive frequency (40.5%) was also found in Manaus, while in Mâncio Lima, Guajará and Oiapoque, the frequencies were low (23.5%, 25% and 22.2% respectively). We also observed the Q87K mutation in a predicted B-cell epitope of pvs25, with no significant changes on its putative antigenicity. Our data suggest that the pvs25 gene is conserved among isolates from different Brazilian Amazon geographic regions, an important observation considering the antigen potentiality as a vaccine candidate to cover distinct P. vivax endemic areas worldwide.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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