| Autor: |
Mohan T; Laboratory for Characterisation and Processing of Polymers, Faculty of Mechanical Engineering , University of Maribor , Smetanova ulica17 , 2000 Maribor , Slovenia., Nagaraj C; Ludwig Boltzmann Institute for Lung Vascular Research , Stiftingtalstrasse 24 , 8010 Graz , Austria., Nagy BM; Ludwig Boltzmann Institute for Lung Vascular Research , Stiftingtalstrasse 24 , 8010 Graz , Austria., Bračič M; Laboratory for Characterisation and Processing of Polymers, Faculty of Mechanical Engineering , University of Maribor , Smetanova ulica17 , 2000 Maribor , Slovenia., Maver U; Faculty of Medicine, Institute of Biomedical Sciences , University of Maribor , Taborska Ulica 8 , SI-2000 Maribor , Slovenia., Olschewski A; Ludwig Boltzmann Institute for Lung Vascular Research , Stiftingtalstrasse 24 , 8010 Graz , Austria.; Chair of Physiology , Otto Loewi Research Center , Neue Stiftingtalstraße 6/D05 , 8010 Graz , Austria., Stana Kleinschek K; Laboratory for Characterisation and Processing of Polymers, Faculty of Mechanical Engineering , University of Maribor , Smetanova ulica17 , 2000 Maribor , Slovenia., Kargl R; Laboratory for Characterisation and Processing of Polymers, Faculty of Mechanical Engineering , University of Maribor , Smetanova ulica17 , 2000 Maribor , Slovenia. |
| Abstrakt: |
This work describes the interaction of the human blood plasma proteins albumin, fibrinogen, and γ-globulins with micro- and nanopatterned polymer interfaces. Protein adsorption studies were correlated with the fibrin clotting time of human blood plasma and with the growth of primary human pulmonary artery endothelial cells (hECs) on these patterns. It was observed that blends of polycaprolactone (PCL) and trimethylsilyl-protected cellulose form various thin-film patterns during spin coating, depending on the mass ratio of the polymers in the spinning solutions. Vapor-phase acid-catalyzed deprotection preserves these patterns but yields interfaces that are composed of hydrophilic cellulose domains enclosed by hydrophobic PCL. The blood plasma proteins are repelled by the cellulose domains, allowing for a suggested selective protein deposition on the PCL domains. An inverse proportional correlation is observed between the amount of cellulose present in the films and the mass of irreversibly adsorbed proteins. This results in significantly increased fibrin clotting times and lower masses of deposited clots on cellulose-containing films as revealed by quartz crystal microbalance with dissipation measurements. Cell viability of hECs grown on these surfaces was directly correlated with higher protein adsorption and faster clot formation. The results show that presented patterned polymer composite surfaces allow for a controllable blood plasma protein coagulation and a significant biological response from hECs. It is proposed that this knowledge can be utilized in regenerative medicine, cell cultures, and artificial vascular grafts by a careful choice of polymers and patterns. |
