Urban airborne particle exposure impairs human lung and blood Mycobacterium tuberculosis immunity.

Autor:	Torres M; Department of Microbiology, Instituto Nacional de Enfermedades Respiratorias, Mexico City, Mexico., Carranza C; Department of Microbiology, Instituto Nacional de Enfermedades Respiratorias, Mexico City, Mexico., Sarkar S; Environmental and Occupational Health, Rutgers School of Public Health New Brunswick Campus, Piscataway, New Jersey, USA., Gonzalez Y; Department of Microbiology, Instituto Nacional de Enfermedades Respiratorias, Mexico City, Mexico., Osornio Vargas A; Paediatrics, University of Alberta, Edmonton, Alberta, Canada., Black K; Environmental and Occupational Health Sciences Institute, Rutgers, The State University of New Jersey, New Brunswick, New Jersey, USA., Meng Q; Environmental and Occupational Health, Rutgers School of Public Health New Brunswick Campus, Piscataway, New Jersey, USA., Quintana-Belmares R; Investigacion basica, Instituto Nacional de Cancerologia, Mexico City, Mexico., Hernandez M; Contaminacion y salud ambiental, Instituto Nacional de Ecologia y Cambio Climatico, Coyoacan, Mexico., Angeles Garcia JJF; Contaminacion y salud ambiental, Instituto Nacional de Ecologia y Cambio Climatico, Coyoacan, Mexico., Páramo-Figueroa VH; Contaminacion y salud ambiental, Instituto Nacional de Ecologia y Cambio Climatico, Coyoacan, Mexico., Iñiguez-Garcia MA; Department of Thoracic Surgery, Instituto Nacional de Enfermedades Respiratorias, Mexico City, Mexico., Flores JL; Departamento de ciencias de la salud, Universidad Autonoma Metropolitana Iztapalapa, Iztapalapa, Mexico., Zhang JJ; Duke Global Health Institute and Nicholas School of the Environment, Duke University, Durham, North Carolina, USA., Gardner CR; Ernest Mario School of Pharmacy, Rutgers University, Piscataway, New Jersey, USA., Ohman-Strickland P; Biostatistics, Rutgers School of Public Health New Brunswick Campus, Piscataway, New Jersey, USA., Schwander S; Urban-Global Public Health and Environmental and Occupational Health, Rutgers School of Public Health New Brunswick Campus, Piscataway, New Jersey, USA schwansk@sph.rutgers.edu.
Jazyk:	angličtina
Zdroj:	Thorax [Thorax] 2019 Jul; Vol. 74 (7), pp. 675-683. Date of Electronic Publication: 2019 Apr 29.
DOI:	10.1136/thoraxjnl-2018-212529
Abstrakt:	Rationale: Associations between urban (outdoor) airborne particulate matter (PM) exposure and TB and potential biological mechanisms are poorly explored. Objectives: To examine whether in vivo exposure to urban outdoor PM in Mexico City and in vitro exposure to urban outdoor PM 2.5 (< 2.5 µm median aerodynamic diameter) alters human host immune cell responses to Mycobacterium tuberculosis . Methods: Cellular toxicity (flow cytometry, proliferation assay (MTS assay)), M. tuberculosis and PM 2.5 phagocytosis (microscopy), cytokine-producing cells (Enzyme-linked immune absorbent spot (ELISPOT)), and signalling pathway markers (western blot) were examined in bronchoalveolar cells (BAC) and peripheral blood mononuclear cells (PBMC) from healthy, non-smoking, residents of Mexico City (n=35; 13 female, 22 male). In vivo-acquired PM burden in alveolar macrophages (AM) was measured by digital image analysis. Measurements and Main Results: In vitro exposure of AM to PM 2.5 did not affect M. tuberculosis phagocytosis. High in vivo-acquired AM PM burden reduced constitutive, M. tuberculosis and PM-induced interleukin-1β production in freshly isolated BAC but not in autologous PBMC while it reduced constitutive production of tumour necrosis factor-alpha in both BAC and PBMC. Further, PM burden was positively correlated with constitutive, PM, M. tuberculosis and purified protein derivative (PPD)-induced interferon gamma (IFN-γ) in BAC, and negatively correlated with PPD-induced IFN-γ in PBMC. Conclusions: Inhalation exposure to urban air pollution PM impairs important components of the protective human lung and systemic immune response against M. tuberculosis . PM load in AM is correlated with altered M. tuberculosis -induced cytokine production in the lung and systemic compartments. Chronic PM exposure with high constitutive expression of proinflammatory cytokines results in relative cellular unresponsiveness. Competing Interests: Competing interests: None declared. (© Author(s) (or their employer(s)) 2019. No commercial re-use. See rights and permissions. Published by BMJ.)
Databáze:	MEDLINE
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