Comparison of histochemical staining techniques for detecting mast cells in oral lesions.

Autor: Mutsaddi S; Department of Oral Pathology and Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre , Belgaum , India., Kotrashetti VS; Department of Oral Pathology and Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre , Belgaum , India., Nayak RS; Department of Oral Pathology and Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre , Belgaum , India., Pattanshetty SM; Department of Oral Pathology and Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre , Belgaum , India.
Jazyk: angličtina
Zdroj: Biotechnic & histochemistry : official publication of the Biological Stain Commission [Biotech Histochem] 2019 Aug; Vol. 94 (6), pp. 459-468. Date of Electronic Publication: 2019 Apr 15.
DOI: 10.1080/10520295.2019.1597986
Abstrakt: Mast cells are large cells with granular cytoplasm that participate in wound healing, angiogenesis and defense against pathogens. They also contribute to inflammation by initiating innate and acquired immunity. The granules of these cells exhibit characteristic staining properties. We investigated toluidine blue, astra blue, Alcian blue-pyronin Y and May-Grunwald Giemsa stains for mast cells in various oral lesions and assessed the efficacy of each for identifying mast cells. Sections were obtained from 10 each of diagnosed cases of inflammatory fibrous hyperplasia, periapical cyst, mild dysplasia, oral submucous fibrosis and oral squamous cell carcinoma and stained using the stains listed above. Mast cells were assessed for their presence, contrast of the mast cell in the connective tissue background and number. We found that May-Grunwald Giemsa stain was the best for identification of mast cells, although toluidine blue staining is less time-consuming. Overall we obtained better results using May-Grunwald Giemsa and toluidine blue for staining mast cells.
Databáze: MEDLINE
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