Incidence of antimicrobial resistance genes and class 1 integron gene cassettes in multidrug-resistant motile Aeromonas sp. isolated from ornamental guppy (Poecilia reticulata).

Autor: Hossain S; Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea., De Silva BCJ; Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea., Wickramanayake MVKS; Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea., Dahanayake PS; Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea., Wimalasena SHMP; Bio-Safety Research Institute and College of Veterinary Medicine, Chonbuk National University, Jeonju, Korea., Heo GJ; Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
Jazyk: angličtina
Zdroj: Letters in applied microbiology [Lett Appl Microbiol] 2019 Jul; Vol. 69 (1), pp. 2-10. Date of Electronic Publication: 2019 May 02.
DOI: 10.1111/lam.13162
Abstrakt: Aeromonas sp. are opportunistic pathogenic bacteria which are associated with various diseases in ornamental fish, aquaculture raised species and wild fisheries. In our study, antimicrobial resistance patterns, antimicrobial resistance genes and class 1 integron gene cassettes of 52 guppy-borne Aeromonas sp. were examined. The isolates were identified as A. veronii (n = 34), A. dhakensis (n = 10), A. hydrophila (n = 3), A. caviae (n = 3) and A. enteropelogenes (n = 2) by gyrB gene sequencing. Every isolate was resistant to at least four antimicrobials in disc diffusion test. The resistance to amoxicillin, nalidixic acid and oxytetracycline was 100% among the tested isolates. 92·30, 76·92, 71·15, 51·92, 51·92 and 50·00% of the isolates were resistant to ampicillin, rifampicin, imipenem, cephalothin, tetracycline and trimethoprim respectively. The multiple antibiotic resistance index values ranged from 0·28 to 0·67. PCR amplification of antimicrobial resistance genes implied the occurrence of tetracycline resistance (tetA (65·39%), tetE (25·00%) and tetB (15·38%)), plasmid-mediated quinolone resistance (qnrS (26·92%) and qnrB (17·31%)) and aminoglycoside resistance (aphaAI-IAB (7·69%) and aac (6')-Ib (3·84%)) genes in the isolates. The IntI gene was positive for 36·54% of the isolates and four class 1 integron gene cassette profiles (aadA2, qacE2-orfD, aadA2-catB2 and dfrA12-aadA2) were identified. These data suggest that ornamental guppy can be a reservoir of multidrug-resistant Aeromonas sp. which comprise different antimicrobial resistance genes and class 1 integrons. SIGNIFICANCE AND IMPACT OF THE STUDY: Antimicrobial resistance genes and integron gene cassettes of ornamental fish-borne aeromonads are poorly studied. The antimicrobial resistance patterns, antimicrobial resistance genes and class 1 integron gene cassettes of Aeromonas sp. isolated from ornamental guppy were characterized for the first time in Korea. The incidence of different antimicrobial resistance genes and class 1 integron gene cassettes were observed in multidrug-resistant Aeromonas isolates. This result suggests that better management practices are necessary to prevent and address the serious consequences of indiscriminate and inappropriate antimicrobial use, and the distribution of multidrug-resistant bacteria.
(© 2019 The Society for Applied Microbiology.)
Databáze: MEDLINE
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