A highly selective amperometric biosensor array for the simultaneous determination of glutamate, glucose, choline, acetylcholine, lactate and pyruvate.

Autor: Kucherenko DY; Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, NAS of Ukraine, 150 Zabolotnogo str., Kyiv 03148, Ukraine; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64, Volodymyrska Str., Kyiv 01003, Ukraine., Kucherenko IS; Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, NAS of Ukraine, 150 Zabolotnogo str., Kyiv 03148, Ukraine; Department of Mechanical Engineering, Iowa State University, Ames, IA 50011, United States. Electronic address: kucherenko.i.s@gmail.com., Soldatkin OO; Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, NAS of Ukraine, 150 Zabolotnogo str., Kyiv 03148, Ukraine; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64, Volodymyrska Str., Kyiv 01003, Ukraine., Topolnikova YV; Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, NAS of Ukraine, 150 Zabolotnogo str., Kyiv 03148, Ukraine., Dzyadevych SV; Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, NAS of Ukraine, 150 Zabolotnogo str., Kyiv 03148, Ukraine; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64, Volodymyrska Str., Kyiv 01003, Ukraine., Soldatkin AP; Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, NAS of Ukraine, 150 Zabolotnogo str., Kyiv 03148, Ukraine; Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64, Volodymyrska Str., Kyiv 01003, Ukraine.
Jazyk: angličtina
Zdroj: Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2019 Aug; Vol. 128, pp. 100-108. Date of Electronic Publication: 2019 Mar 31.
DOI: 10.1016/j.bioelechem.2019.03.010
Abstrakt: The work was aimed at the development of a biosensor array for the simultaneous determination of six solutes (glutamate, glucose, choline, acetylcholine, lactate, and pyruvate) in aqueous solutions. Enzymes selective for these substrates were immobilized on the surface of amperometric platinum disc electrodes and served as bioselective elements of a biosensor array. Direct enzymatic analysis by the developed biosensors provided high sensitivity to the tested substrates (limits of detection were 1-5 μM). The linear ranges of the biosensors were from 0.001-0.01 mM to 0.2-2.5 mM. The influence of solution pH, ionic strength and buffer capacity on the biosensor responses was investigated; the conditions for simultaneous operation of all the bioselective elements were optimized. The absence of any cross-influence of the substrates of enzymatic systems used was shown as well as a high selectivity of the biosensors and the absence of any impact of interfering substances (ascorbic acid, dopamine, cysteine, paracetamol). The developed biosensor array had good response reproducibility and storage stability. The array is suitable for rapid (0.5-1 min) and simple simultaneous determination of glutamate, glucose, choline, acetylcholine, lactate, and pyruvate in aqueous (biological) samples; furthermore, the creation of a single chip with six sensitive elements is possible as well as the addition of other biosensors.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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