Molecular analysis of clonally related Salmonella Typhi recovered from epidemiologically unrelated cases of typhoid fever, Brazil.
| Autor: | Tiba-Casas MR; Instituto Adolfo Lutz, São Paulo, Brazil. Electronic address: monique.casas@ial.sp.gov.br., Sacchi CT; Instituto Adolfo Lutz, São Paulo, Brazil., Gonçalves CR; Instituto Adolfo Lutz, São Paulo, Brazil., Almeida EA; Instituto Adolfo Lutz, São Paulo, Brazil., Soares FB; Instituto Adolfo Lutz, São Paulo, Brazil., de Jesus Bertani AM; Instituto Adolfo Lutz, São Paulo, Brazil., Fernandes SA; Instituto Adolfo Lutz, São Paulo, Brazil., de Paula Eduardo MB; Coordenadoria de Controle de Doenças, Centro de Vigilância Epidemiológica Prof. Alexandre Vranjac, São Paulo, Brazil., Camargo CH; Instituto Adolfo Lutz, São Paulo, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases [Int J Infect Dis] 2019 Apr; Vol. 81, pp. 191-195. Date of Electronic Publication: 2019 Mar 05. |
| DOI: | 10.1016/j.ijid.2019.02.009 |
| Abstrakt: | Background: The primary method of molecular subtyping for the identification and investigation of outbreaks has been pulsed-field gel electrophoresis (PFGE). In some cases, this technique has not been able to show discrimination between the unrelated strains that can be achieved by whole genome sequencing (WGS). Methods: The aim of this study was to determine the strengths and drawbacks of WGS using different analytic approaches compared to traditional typing method, PFGE, for retrospectively typing clusters cases of 28 S. Typhi. Results: We evaluated three analytical approaches on the WGS data set (Nucleotide Difference (ND), (SNPs) and Whole genome multi locus sequence typing (wgMLST) that identically classified the clusters-related strains into two clusters, cluster A (with strains from 2017), and Cluster B (with strains from 2007). Conclusions: In this study WGS based typing, was able to compete with PFGE for differentiation of the clusters of S. Typhi strains. (Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| Databáze: | MEDLINE |
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