SELfies and CELLfies: Whole Genome Sequencing and Annotation of Five Antibiotic Resistant Bacteria Isolated from the Surfaces of Smartphones, An Inquiry Based Laboratory Exercise in a Genomics Undergraduate Course at the Rochester Institute of Technology.

Autor:	Parthasarathy A; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA., Wong NH; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA., Weiss AN; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA., Tian S; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA., Ali SE; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA., Cavanaugh NT; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA., Chinsky TM; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA., Cramer CE; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA., Gupta A; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA., Jha R; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA., Johnson LK; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA., Tuason ED; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA., Klafehn LM; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA., Krishnadas V; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA., Musich RJ; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA., Pfaff JM; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA., Richman SC; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA., Shumway AJ; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA., Hudson AO; The Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester NY, USA.
Jazyk:	angličtina
Zdroj:	Journal of genomics [J Genomics] 2019 Feb 19; Vol. 7, pp. 26-30. Date of Electronic Publication: 2019 Feb 19 (Print Publication: 2019).
DOI:	10.7150/jgen.31911
Abstrakt:	Are touchscreen devices a public health risk for the transmission of pathogenic bacteria, especially those that are resistant to antibiotics? To investigate this, we embarked on a project aimed at isolating and identifying bacteria that are resistant to antibiotics from the screens of smartphones. Touchscreen devices have become ubiquitous in society, and it is important to evaluate the potential risks they pose towards public health, especially as it pertains to the harboring and transmission of pathogenic bacteria that are resistant to antibiotics. Sixteen bacteria were initially isolated of which five were unique (four Staphylococcus species and one Micrococcus species). The genomes of the five unique isolates were subsequently sequenced and annotated. The genomes were analyzed using in silico tools to predict the synthesis of antibiotics and secondary metabolites using the anti biotics and S econdary M etabolite A nalysis SH ell (antiSMASH) tool in addition to the presence of gene clusters that denote resistance to antibiotics using the R esistance G ene I dentifier (RGI) tool. In vivo analysis was also done to assess resistance/susceptibility to four antibiotics that are commonly used in a research laboratory setting. The data presented in this manuscript is the result of a semester-long inquiry based laboratory exercise in the genomics course (BIOL340) in the Thomas H. Gosnell School of Life Sciences/College of Science at the Rochester Institute of Technology. Competing Interests: Competing Interests: The authors have declared that no competing interest exists.
Databáze:	MEDLINE
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