| Autor: |
Killiny N; University of Florida, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL, USA. nabilkilliny@ufl.edu., Etxeberria E; University of Florida, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL, USA., Flores AP; Universidad Nacional Autonoma de Mexico, Fac. De Ciencias, Universidad 3000, Circuito Exterior S/N, Distrito Federal, 04510, Mexico., Blanco PG; University of Florida, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL, USA., Reyes TF; University of Florida, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL, USA.; Instituto Politecnico Nacional, CICATA, Carretera Tampico-Puerto Industrial Altamira Km 14.5, Industrial Altamira, 89600, Altamira, Tampico, Mexico., Cabrera LP; University of Florida, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, FL, USA.; Instituto Politecnico Nacional, CICATA, Carretera Tampico-Puerto Industrial Altamira Km 14.5, Industrial Altamira, 89600, Altamira, Tampico, Mexico. |
| Abstrakt: |
To prevent the spread of diseases in humans, animals or plants, determining whether potential vectors are infected is crucial. For example, early detection of the citrus disease Huanglongbing, which has been a scourge on the citrus industries around the world, is a critical need. This vector-borne disease is transmitted by Diaphorina citri, the Asian citrus psyllid, which carries the putative bacterial phytopathogen, Candidatus Liberibacter asiaticus (CLas). In this investigation, we introduced Laser-Induced Breakdown Spectroscopy (LIBS) to reveal key biochemical differences between CLas-infected and non-infected psyllids. The emission spectra captured from laser ablation of CLas-infected and healthy psyllids were processed through the principal component analysis (PCA) method and compared. Thirteen peaks from seven different elements were detected in D. citri. The t-test showed that CLas-infected D. citri were deficients in zinc, iron, copper, magnesium, calcium, and nitrogen. The PCA showed that LIBS can successfully differentiate between CLas-infected and healthy D. citri by comparing their elemental profile. In this work, we demonstrated a method that allows for a fast and precise compositional microanalysis of an insect vector which can contribute to the early detection of citrus huanglongbing. |
