| Autor: |
Santos MFM; Hospital Israelita Albert Einstein, São Paulo, SP, Brasil., Oliveira FCAC; Hospital Israelita Albert Einstein, São Paulo, SP, Brasil., Kishimoto RK; Hospital Israelita Albert Einstein, São Paulo, SP, Brasil., Borri D; Hospital Israelita Albert Einstein, São Paulo, SP, Brasil., Santos FPS; Hospital Israelita Albert Einstein, São Paulo, SP, Brasil., Campregher PV; Hospital Israelita Albert Einstein, São Paulo, SP, Brasil., Silveira PAA; Hospital Israelita Albert Einstein, São Paulo, SP, Brasil., Hamerschlak N; Hospital Israelita Albert Einstein, São Paulo, SP, Brasil., Mangueira CLP; Hospital Israelita Albert Einstein, São Paulo, SP, Brasil., Duarte FB; Hospital Universitário Walter Cantídio, Universidade Federal do Ceará, Fortaleza, CE, Brasil., Crepaldi AH; Hospital de Câncer de Mato Grosso, Cuiabá, MT, Brasil., Salvino MA; Hospital São Rafael/Monte Tabor, Salvador, BA, Brasil., Velloso EDRP; Hospital Israelita Albert Einstein, São Paulo, SP, Brasil.; Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brasil. |
| Abstrakt: |
Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), myeloproliferative neoplasms (n=137), and other with conclusive diagnosis (n=6), and reactive disorders/no conclusive diagnosis (n=75). The rate of unsuccessful karyotyping was 18.5% and was associated with the use of PB and a low number of nucleated cells (≤7×103/µL) in the sample. High and low cellularity in BM and high and low cellularity in PB samples showed no metaphases in 3.9, 39.7, 41.9, and 84.6% of cases, respectively. Collecting a good BM sample is the key for the success of karyotyping in MN and avoids the use of expensive molecular techniques. |
