[Clinical trials of salmonella enrichment medium.]
Autor: | Shepelin AP; FederalState Institution of Science State Research Center for Applied Microbiology & Biotechnology, Rospotrebnadzor, 142279, Obolensk, Serpukhov district, Moscow region, Russian Federation., Polosenko OV; FederalState Institution of Science State Research Center for Applied Microbiology & Biotechnology, Rospotrebnadzor, 142279, Obolensk, Serpukhov district, Moscow region, Russian Federation., Marchikhina II; FederalState Institution of Science State Research Center for Applied Microbiology & Biotechnology, Rospotrebnadzor, 142279, Obolensk, Serpukhov district, Moscow region, Russian Federation., Sholokhova LP; FederalState Institution of Science State Research Center for Applied Microbiology & Biotechnology, Rospotrebnadzor, 142279, Obolensk, Serpukhov district, Moscow region, Russian Federation., Azhermacheva NI; FederalState Institution of Science State Research Center for Applied Microbiology & Biotechnology, Rospotrebnadzor, 142279, Obolensk, Serpukhov district, Moscow region, Russian Federation., Ershova MG; State budgetary institution of health care Infectious disease hospital, 150040 Yaroslavl, Russia., Poletaeva ED; State budgetary institution of health care Infectious disease hospital, 150040 Yaroslavl, Russia. |
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Jazyk: | ruština |
Zdroj: | Klinicheskaia laboratornaia diagnostika [Klin Lab Diagn] 2018; Vol. 63 (9), pp. 557-563. |
DOI: | 10.18821/0869-2084-2018-63-9-557-563 |
Abstrakt: | Salmonella infections continue to be a serious problem in modern medicine. Being intestinal infections-associated pathogens, representatives of the genus Salmonella manifest themselves as pathogenic bacteria, especially in developing nosocomial infections. Given the polymorphism of clinical symptoms of salmonellosis, laboratory studies using bacteriological and serological methods are an important link in the diagnosis. In addition, the general prevention of salmonellosis includes measures to identify bacteria carriers, to ensure control over the incidence in farm animals and birds, food safety, etc. The list of nutrient media to isolate and identify Salmonella is lengthy and steadily extending, and the choice of specific media is largely relies on the nature of the material under study as well as on the idea of the potential availability of Salmonella bacteria in it, with research, diagnosis or epidemic situation being taken into account. The SRCAMB (Rospotrebnadzor) has designed two nutrient media allowing the enrichment and isolation Salmonella from various clinical samples. These are "Nutrient Medium for Enrichment of Salmonella, Dry (Magnesium medium) and "Nutrient Agar with Brilliant Green, Phenolic Red, Lactose and Sucrose, Dry (BPLS-FMH agar)." Growth and inhibitory properties of the new culture media produced by the SRCAMB and commercial domestic and foreign counterparts have been compared by using clinical material. Domestic nutrient media such as Magnesium medium and BPLS-FMH agar were proved to correspond to their commercial analogues when being used for enrichment, isolating and counting Salmonella bacteria in clinical specimens to have bacteriological control real data. Competing Interests: The authors declare no conflict of interest. |
Databáze: | MEDLINE |
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