A mobile-phone based high-resolution microendoscope to image cervical precancer.

Autor: Grant BD; Department of Bioengineering, Rice University, Houston, Texas, United States of America., Quang T; Department of Bioengineering, Rice University, Houston, Texas, United States of America., Possati-Resende JC; Department of Cancer Prevention, Pio XII Foundation, Barretos Cancer Hospital, Barretos, Brazil., Scapulatempo-Neto C; Molecular Oncology Research Center, Pio XII Foundation, Barretos Cancer Hospital, Barretos, Brazil.; Department of Pathology, Pio XII Foundation, Barretos Cancer Hospital, Barretos, Brazil., de Macedo Matsushita G; Department of Pathology, Pio XII Foundation, Barretos Cancer Hospital, Barretos, Brazil., Mauad EC; Department of Cancer Prevention, Pio XII Foundation, Barretos Cancer Hospital, Barretos, Brazil., Stoler MH; Department of Pathology, University of Virginia School of Medicine, Charlottesville, VA, United States of America., Castle PE; Global Coalition against Cervical Cancer, Arlington, VA, United States of America.; Department of Epidemiology and Population Health, Albert Einstein College of Medicine, Bronx, NY, United States of America., Guerreiro Fregnani JHT; Center for the Researcher Support, Pio XII Foundation, Barretos Cancer Hospital, Barretos, Brazil., Schmeler KM; Department of Gynecologic Oncology and Reproductive Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX, United States of America., Richards-Kortum R; Department of Bioengineering, Rice University, Houston, Texas, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2019 Feb 06; Vol. 14 (2), pp. e0211045. Date of Electronic Publication: 2019 Feb 06 (Print Publication: 2019).
DOI: 10.1371/journal.pone.0211045
Abstrakt: Nearly 90% of cervical cancer cases and deaths occur in low- and middle-income countries that lack comprehensive national HPV immunization and cervical cancer screening programs. In these settings, it is difficult to implement screening programs due to a lack of infrastructure and shortage of trained personnel. Screening programs based on visual inspection with acetic acid (VIA) have been successfully implemented in some low-resource settings. However, VIA has poor specificity and up to 90% of patients receiving treatment based on a positive VIA exam are over-treated. A number of studies have suggested that high-resolution cervical imaging to visualize nuclear morphology in vivo can improve specificity by better distinguishing precancerous and benign lesions. To enable high-resolution imaging in low-resource settings, we developed a portable, low-cost, high-resolution microendoscope that uses a mobile phone to detect and display images of cervical epithelium in vivo with subcellular resolution. The device was fabricated for less than $2,000 using commercially available optical components including filters, an LED and triplet lenses assembled in a 3D-printed opto-mechanical mount. We show that the mobile high-resolution microendoscope achieves similar resolution and signal-to-background ratio as previously reported high-resolution microendoscope systems using traditional cameras and computers to detect and display images. Finally, we demonstrate the ability of the mobile high-resolution microendoscope to image normal and precancerous squamous epithelium of the cervix in vivo in a gynecological referral clinic in Barretos, Brazil.
Competing Interests: I have read the journal's policy and the authors of this manuscript have the following competing interests: Phil Castle: I have received HPV tests for research at a reduced or no cost from Roche, Becton Dickinson, Cepheid, and Arbor Vita Corporation. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
Databáze: MEDLINE
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