Top-Down Garbage Collector: a tool for selecting high-quality top-down proteomics mass spectra.

Autor:	Lima DB; Mass Spectrometry for Biology Unit, CNRS USR 2000, Institut Pasteur, Paris, France., Silva ARF; Laboratory for Structural and Computational Proteomics, Carlos Chagas Institute, Fiocruz, Paraná, Brazil., Dupré M; Mass Spectrometry for Biology Unit, CNRS USR 2000, Institut Pasteur, Paris, France., Santos MDM; Laboratory for Structural and Computational Proteomics, Carlos Chagas Institute, Fiocruz, Paraná, Brazil., Clasen MA; Laboratory for Structural and Computational Proteomics, Carlos Chagas Institute, Fiocruz, Paraná, Brazil., Kurt LU; Laboratory for Structural and Computational Proteomics, Carlos Chagas Institute, Fiocruz, Paraná, Brazil., Aquino PF; Leônidas and Maria Deane Institute, Fiocruz, Amazonas, Brazil., Barbosa VC; Systems Engineering and Computer Science Program, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Carvalho PC; Laboratory for Structural and Computational Proteomics, Carlos Chagas Institute, Fiocruz, Paraná, Brazil., Chamot-Rooke J; Mass Spectrometry for Biology Unit, CNRS USR 2000, Institut Pasteur, Paris, France.
Jazyk:	angličtina
Zdroj:	Bioinformatics (Oxford, England) [Bioinformatics] 2019 Sep 15; Vol. 35 (18), pp. 3489-3490.
DOI:	10.1093/bioinformatics/btz085
Abstrakt:	Motivation: We present the first tool for unbiased quality control of top-down proteomics datasets. Our tool can select high-quality top-down proteomics spectra, serve as a gateway for building top-down spectral libraries and, ultimately, improve identification rates. Results: We demonstrate that a twofold rate increase for two E. coli top-down proteomics datasets may be achievable. Availability and Implementation: http://patternlabforproteomics.org/tdgc, freely available for academic use. Supplementary Information: Supplementary data are available at Bioinformatics online. (© The Author(s) 2019. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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