| Autor: |
Cleophas MCP; Department of Internal Medicine, Radboud university medical center, Nijmegen, The Netherlands.; Radboud Institute for Molecular Life Sciences (RIMLS), Radboud university medical center, Nijmegen, The Netherlands., Ratter JM; Department of Internal Medicine, Radboud university medical center, Nijmegen, The Netherlands.; Radboud Institute for Molecular Life Sciences (RIMLS), Radboud university medical center, Nijmegen, The Netherlands.; Nutrition, Metabolism and Genomics Group, Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands., Bekkering S; Department of Internal Medicine, Radboud university medical center, Nijmegen, The Netherlands.; Radboud Institute for Molecular Life Sciences (RIMLS), Radboud university medical center, Nijmegen, The Netherlands., Quintin J; Immunology of Fungal Infections group, Department of Mycology, Institut Pasteur, Paris, France., Schraa K; Department of Internal Medicine, Radboud university medical center, Nijmegen, The Netherlands., Stroes ES; Department of Vascular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands., Netea MG; Department of Internal Medicine, Radboud university medical center, Nijmegen, The Netherlands.; Radboud Institute for Molecular Life Sciences (RIMLS), Radboud university medical center, Nijmegen, The Netherlands.; Department for Genomics & Immunoregulation, Life and Medical Sciences Institute (LIMES), University of Bonn, 53115, Bonn, Germany., Joosten LAB; Department of Internal Medicine, Radboud university medical center, Nijmegen, The Netherlands. leo.joosten@radboudumc.nl.; Radboud Institute for Molecular Life Sciences (RIMLS), Radboud university medical center, Nijmegen, The Netherlands. leo.joosten@radboudumc.nl.; Department of Medical Genetics, Iuliu Haţieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania. leo.joosten@radboudumc.nl. |
| Abstrakt: |
Sodium butyrate is well-known for its immune-modulatory properties. Studies until now only focused on the in vitro effects of butyrate or assessed local effects in the gut upon butyrate administration. In this trial, we studied the systemic anti-inflammatory effects induced by sodium butyrate supplementation in humans. Nine healthy (Lean) and ten obese (metabolic syndrome group, MetSyn) males were given 4 grams sodium butyrate daily for 4 weeks. PBMCs were isolated before and after supplementation for direct stimulation experiments and induction of trained immunity by oxidized low-density lipoprotein (oxLDL), β-glucan, or Bacillus Calmette-Guérin vaccine (BCG). Butyrate supplementation moderately affected some of the cytokine responses in the MetSyn group. In the direct stimulation setup, effects of butyrate supplementation were limited. Interestingly, butyrate supplementation decreased oxLDL-induced trained immunity in the MetSyn group for LPS-induced IL-6 responses and Pam3CSK4-induced TNF-α responses. Induction of trained immunity by β-glucan was decreased by butyrate in the MetSyn group for Pam3CSK4-induced IL-10 production. In this study, while having only limited effects on the direct stimulation of cytokine production, butyrate supplementation significantly affected trained immunity in monocytes of obese individuals with metabolic complications. Therefore, oral butyrate supplementation may be beneficial in reducing the overall inflammatory status of circulating monocytes in patients with metabolic syndrome. |
