Naturally-Derived Biphasic Calcium Phosphates through Increased Phosphorus-Based Reagent Amounts for Biomedical Applications.

Autor: Mocanu AC; Department of Metallic Materials Science, Physical Metallurgy, University Politehnica of Bucharest, 313 Splaiul Independentei, J Building, District 6, 060042 Bucharest, Romania. mcn_aura@hotmail.com.; Department of Research, Development and Innovation, S.C. Nuclear NDT Research & Services S.R.L, 104 Berceni Str., Central Laboratory Building, District 4, 041919 Bucharest, Romania. mcn_aura@hotmail.com., Stan GE; National Institute of Materials Physics, Laboratory of Multifunctional Materials and Structures, 405A Atomistilor Str., 077125 Măgurele-Ilfov, Romania. george_stan@infim.ro., Maidaniuc A; Department of Metallic Materials Science, Physical Metallurgy, University Politehnica of Bucharest, 313 Splaiul Independentei, J Building, District 6, 060042 Bucharest, Romania. andreea.maidaniuc@gmail.com.; Destructive and Nondestructive Testing Laboratory,S.C. Nuclear NDT Research & Services S.R.L, 104 Berceni Str., Central Laboratory Building, District 4, 041919 Bucharest, Romania. andreea.maidaniuc@gmail.com., Miculescu M; Department of Metallic Materials Science, Physical Metallurgy, University Politehnica of Bucharest, 313 Splaiul Independentei, J Building, District 6, 060042 Bucharest, Romania. m_miculescu@yahoo.com., Antoniac IV; Department of Metallic Materials Science, Physical Metallurgy, University Politehnica of Bucharest, 313 Splaiul Independentei, J Building, District 6, 060042 Bucharest, Romania. antoniac.iulian@gmail.com., Ciocoiu RC; Department of Metallic Materials Science, Physical Metallurgy, University Politehnica of Bucharest, 313 Splaiul Independentei, J Building, District 6, 060042 Bucharest, Romania. ciocoiurobert@gmail.com., Voicu ȘI; Department of Analytical Chemistry and Environmental Engineering, University Politehnica of Bucharest, 1-7 Gh. Polizu Str., Polizu campus, L 015 Building, District 1, 011061 Bucharest, Romania. svoicu@gmail.com., Mitran V; Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania. valentinamitran@yahoo.com., Cîmpean A; Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095 Bucharest, Romania. anisoara.cimpean@bio.unibuc.ro., Miculescu F; Department of Metallic Materials Science, Physical Metallurgy, University Politehnica of Bucharest, 313 Splaiul Independentei, J Building, District 6, 060042 Bucharest, Romania. f_miculescu@yahoo.com.
Jazyk: angličtina
Zdroj: Materials (Basel, Switzerland) [Materials (Basel)] 2019 Jan 25; Vol. 12 (3). Date of Electronic Publication: 2019 Jan 25.
DOI: 10.3390/ma12030381
Abstrakt: Calcium carbonate from marble and seashells is an eco-friendly, sustainable, and largely available bioresource for producing natural bone-like calcium phosphates (CaPs). Based on three main objectives, this research targeted the: (i) adaptation of an indirect synthesis route by modulating the amount of phosphorus used in the chemical reaction, (ii) comprehensive structural, morphological, and surface characterization, and (iii) biocompatibility assessment of the synthesized powdered samples. The morphological characterization was performed on digitally processed scanning electron microscopy (SEM) images. The complementary 3D image augmentation of SEM results also allowed the quantification of roughness parameters. The results revealed that both morphology and roughness were modulated through the induced variation of the synthesis parameters. Structural investigation of the samples was performed by Fourier transform infrared spectroscopy and X-ray diffraction. Depending on the phosphorus amount from the chemical reaction, the structural studies revealed the formation of biphasic CaPs based on hydroxyapatite/brushite or brushite/monetite. The in vitro assessment of the powdered samples demonstrated their capacity to support MC3T3-E1 pre-osteoblast viability and proliferation at comparable levels to the negative cytotoxicity control and the reference material (commercial hydroxyapatite). Therefore, these samples hold great promise for biomedical applications.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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