Adenovirus 5 produces obesity and adverse metabolic, morphological, and functional changes in the long term in animals fed a balanced diet or a high-fat diet: a study on hamsters.

Autor: Montes-Galindo DA; Department of Molecular Medicine, School of Medicine, University of Colima, Avenue Universidad 333, Colonia Las Víboras, CP 28040, Colima, Mexico.; Department of Research, Cancerology State Institute,Colima State Health Services, 28085, Colima, Mexico., Espiritu-Mojarro AC; Department of Research, Cancerology State Institute,Colima State Health Services, 28085, Colima, Mexico.; Department of Research, Mexican Social Security Institute, Villa de Alvarez, 28983, Colima, Mexico., Melnikov V; Department of Molecular Medicine, School of Medicine, University of Colima, Avenue Universidad 333, Colonia Las Víboras, CP 28040, Colima, Mexico., Moy-López NA; Laboratory of Neuroscience, School of Psychology, University of Colima, 28040, Colima, Mexico., Soriano-Hernandez AD; Department of Molecular Medicine, School of Medicine, University of Colima, Avenue Universidad 333, Colonia Las Víboras, CP 28040, Colima, Mexico.; Department of Research, Cancerology State Institute,Colima State Health Services, 28085, Colima, Mexico., Galvan-Salazar HR; Department of Research, Cancerology State Institute,Colima State Health Services, 28085, Colima, Mexico.; Department of Research, Mexican Social Security Institute, Villa de Alvarez, 28983, Colima, Mexico., Guzman-Muñiz J; Laboratory of Neuroscience, School of Psychology, University of Colima, 28040, Colima, Mexico., Guzman-Esquivel J; Department of Research, Mexican Social Security Institute, Villa de Alvarez, 28983, Colima, Mexico., Martinez-Fierro ML; Molecular Medicine Laboratory, Academic Unit of Human Medicine and Health Sciences, Zacatecas Autonomous University, 98160, Zacatecas, Mexico., Rodriguez-Sanchez IP; Molecular and Structural Physiology Laboratory, School of Biological Sciences, Universidad Autónoma de Nuevo León, 66450, Monterrey, Nuevo León, Mexico., Paz-Michel B; Esteripharma Mexico, 03100, Mexico City, Mexico., Zaizar-Fregoso SA; Department of Molecular Medicine, School of Medicine, University of Colima, Avenue Universidad 333, Colonia Las Víboras, CP 28040, Colima, Mexico.; Department of Research, Cancerology State Institute,Colima State Health Services, 28085, Colima, Mexico., Sanchez-Ramirez CA; Department of Molecular Medicine, School of Medicine, University of Colima, Avenue Universidad 333, Colonia Las Víboras, CP 28040, Colima, Mexico., Ramirez-Flores M; Department of Molecular Medicine, School of Medicine, University of Colima, Avenue Universidad 333, Colonia Las Víboras, CP 28040, Colima, Mexico., Delgado-Enciso I; Department of Molecular Medicine, School of Medicine, University of Colima, Avenue Universidad 333, Colonia Las Víboras, CP 28040, Colima, Mexico. ivan_delgado_enciso@ucol.mx.; Department of Research, Cancerology State Institute,Colima State Health Services, 28085, Colima, Mexico. ivan_delgado_enciso@ucol.mx.
Jazyk: angličtina
Zdroj: Archives of virology [Arch Virol] 2019 Mar; Vol. 164 (3), pp. 775-786. Date of Electronic Publication: 2019 Jan 21.
DOI: 10.1007/s00705-018-04132-6
Abstrakt: Adenovirus 5 (Ad-5) infection is a common cause of acute respiratory infections and the main vector used in gene therapy. There are few studies on the relationship of Ad-5 to obesity. In the present study, we evaluated the chronic effects of Ad-5 infection on golden (Syrian) hamsters fed either a balanced diet (BD) or a high-fat diet (HFD). After a single inoculation with Ad-5 (1 × 10 7 pfu), the body weight of the animals was measured weekly. Medium-term (22 weeks) serum biochemical analyses and long-term (44 weeks) liver morphology, adiposity, and locomotive functionality (movement velocity) assessments were carried out. In the animals fed the BD, adenovirus infection produced hyperglycemia and hyperlipidemia. In the long term, it produced a 57% increase in epididymal pad fat and a 30% body weight gain compared with uninoculated animals. In addition, morphological changes related to non-alcoholic fatty liver disease (NAFLD) were observed. The animals fed the HFD had similar but more severe changes. In addition, the hamsters presented an obesity paradox: at the end of the study, the animals that had the most morphological and functional changes (significantly reduced movement velocity) had the lowest body weight. Despite the fact that an HFD appears to be a more harmful factor in the long term than adenovirus infection alone, infection could increase the severity of harmful effects in individuals with an HFD. Epidemiological studies are needed to evaluate the effect of adenovirus as a precursor of chronic liver and cardiovascular diseases, including the chronic effects of gene therapy.
Databáze: MEDLINE
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